Mouse Kars ELISA Kit

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  • Alternative name

    Mouse lysyl-tRNA synthetase ELISA Kit;Mouse LysRS ELISA Kit;Mouse AA589550 ELISA Kit;Mouse AL024334 ELISA Kit;Mouse AL033315 ELISA Kit;Mouse AL033367 ELISA Kit;Mouse D8Ertd698e ELISA Kit;Mouse D8Wsu108e ELISA Kit;Mouse mKIAA0070 ELISA Kit;Mouse lysine--tRNA ligase ELISA Kit;

  • Catalog
    E059219
  • species
    Mouse
  • GeneKars
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of KARS. No significant cross-reactivity or interference between KARS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity11.7pg/mL.
  • Intended UseMouse Kars ELISA Kit allows for the in vitro quantitative determination of Kars , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KARS in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to KARS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to KARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Lysine--tRNA ligase Protein information
  • Uniprot ID SYK_MOUSE
  • Uniprot AC Q99MN1;
  • UniGene Mm.196544; Mm.410491;
  • GeneID 85305
  • KEGG mmu:85305;
  • Mouse Lysine--tRNA ligase Protein SEQUENCE
  • SEQUENCE 595 AA; 67840 MW; 055889E87C11AA97 CRC64;

    MATLQESEVK VDGEQKLSKN ELKRRLKAEK KLAEKEAKQK ELSEKQLNQT

    ASAPNHTADN GVGAEEETLD PNQYYKIRSQ AVQQLKVTGE DPYPHKFHVD

    ISLTQFIQEY SHLQPGDHLT DVTLKVAGRI HAKRASGGKL IFYDLRGEGV

    KLQVMANSRN YKSEEEFVHI NNKLRRGDII GVEGNPGKTK KGELSIIPQE

    ITLLSPCLHM LPHLHFGLKD KETRYRQRYL DLILNDFVRQ KFIVRSKIIT

    YIRSFLDELG FLEIETPMMN IIPGGAVAKP FITYHNELDM NLYMRIAPEL

    YHKMLVVGGI DRVYEIGRQF RNEGIDLTHN PEFTTCEFYM AYADYHDLME

    ITEKMLSGMV KSITGSYKIT YHPDGPEGQA YEVDFTPPFR RISMVEELEK

    ALGVKLPETS LFETEETRKI LDDICVAKAV ECPPPRTTAR LLDKLVGEFL

    EVTCISPTFI CDHPQIMSPL AKWHRSKEGL TERFELFVMK KEICNAYTEL

    NDPVRQRQLF EEQAKAKAAG DDEAMFIDEN FCTALEYGLP PTAGWGMGID

    RLTMFLTDSN NIKEVLLFPA MKPEDKKETA ATTETPESTE ASPSV

  • UCSC uc009nne.3; mouse.;



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