Human LYPLA2 ELISA Kit

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  • Alternative name

    Human APT-2 ELISA Kit;Human lysophospholipase II ELISA Kit;Human LPL-II ELISA Kit;Human lysoPLA II ELISA Kit;Human APT2 ELISA Kit;Human DJ886K2.4 ELISA Kit;Human acyl-protein thioesterase 2 ELISA Kit;Human testicular tissue protein Li 3 ELISA Kit;

  • Catalog
    E000592
  • species
    Human
  • GeneLYPLA2
  • Other Species Mouse Lypla2 ELISA KitRat Lypla2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LYPLA2. No significant cross-reactivity or interference between LYPLA2and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LYPLA2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LYPLA2 ELISA Kit allows for the in vitro quantitative determination of LYPLA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses||This LYPLA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LYPLA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: LYPLA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LYPLA2 antibody and an LYPLA2-HRP conjugate. The assay sample and buffer are incubated together with LYPLA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theLYPLA2 concentration since LYPLA2 from samples and LYPLA2-HRP conjugate compete for the anti-LYPLA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LYPLA2 from the sample, fewer sites are left to bind LYPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LYPLA2 concentration in each sample is interpolated from this standard curve.
  • Human Acyl-protein thioesterase 2 Protein information
  • Uniprot ID LYPA2_HUMAN
  • Uniprot AC O95372; Q7Z4Z2;
  • UniGene Hs.533479;
  • GeneID 11313
  • KEGG hsa:11313;
  • Human Acyl-protein thioesterase 2 Protein SEQUENCE
  • SEQUENCE 231 AA; 24737 MW; 813C9C71757C5135 CRC64;

    MCGNTMSVPL LTDAATVSGA ERETAAVIFL HGLGDTGHSW ADALSTIRLP

    HVKYICPHAP RIPVTLNMKM VMPSWFDLMG LSPDAPEDEA GIKKAAENIK

    ALIEHEMKNG IPANRIVLGG FSQGGALSLY TALTCPHPLA GIVALSCWLP

    LHRAFPQAAN GSAKDLAILQ CHGELDPMVP VRFGALTAEK LRSVVTPARV

    QFKTYPGVMH SSCPQEMAAV KEFLEKLLPP V

  • UCSC uc001bht.4; human.;




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