Human ADAMTS15 ELISA Kit

Alternative name
Human ADAM-TS 15 ELISA Kit;Human ADAM-TS15 ELISA Kit;Human ADAMTS-15 ELISA Kit;Human ADAM metallopeptidase with thrombospondin type 1 motif 15 ELISA Kit;Human A disintegrin and metalloproteinase with thrombospondin motifs 15 ELISA Kit;Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15, preproprotein ELISA Kit;Human metalloprotease disintegrin 15 with thrombospondin domains ELISA Kit;
Catalog
E000059
species
Human
GeneADAMTS15
Standard Curve
Other Species Mouse Adamts15 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADAMTS15. No significant cross-reactivity or interference between ADAMTS15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS15 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ADAMTS15 ELISA Kit allows for the in vitro quantitative determination of ADAMTS15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: ADAMTS15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS15 antibody and an ADAMTS15-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS15 concentration since ADAMTS15 from samples and ADAMTS15-HRP conjugate compete for the anti-ADAMTS15 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS15 from the sample, fewer sites are left to bind ADAMTS15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS15 concentration in each sample is interpolated from this standard curve.
Human A disintegrin and metalloproteinase with thrombospondin motifs 15 Protein information
Uniprot ID ATS15_HUMAN
Uniprot AC Q8TE58; Q32MI6;
UniGene Hs.534221;
GeneID 170689
KEGG hsa:170689;
Human A disintegrin and metalloproteinase with thrombospondin motifs 15 Protein SEQUENCE
SEQUENCE 950 AA; 103287 MW; 5DFBE18285CCCC3B CRC64;
MLLLGILTLA FAGRTAGGSE PEREVVVPIR LDPDINGRRY YWRGPEDSGD
QGLIFQITAF QEDFYLHLTP DAQFLAPAFS TEHLGVPLQG LTGGSSDLRR
CFYSGDVNAE PDSFAAVSLC GGLRGAFGYR GAEYVISPLP NASAPAAQRN
SQGAHLLQRR GVPGGPSGDP TSRCGVASGW NPAILRALDP YKPRRAGFGE
SRSRRRSGRA KRFVSIPRYV ETLVVADESM VKFHGADLEH YLLTLLATAA
RLYRHPSILN PINIVVVKVL LLRDRDSGPK VTGNAALTLR NFCAWQKKLN
KVSDKHPEYW DTAILFTRQD LCGATTCDTL GMADVGTMCD PKRSCSVIED
DGLPSAFTTA HELGHVFNMP HDNVKVCEEV FGKLRANHMM SPTLIQIDRA
NPWSACSAAI ITDFLDSGHG DCLLDQPSKP ISLPEDLPGA SYTLSQQCEL
AFGVGSKPCP YMQYCTKLWC TGKAKGQMVC QTRHFPWADG TSCGEGKLCL
KGACVERHNL NKHRVDGSWA KWDPYGPCSR TCGGGVQLAR RQCTNPTPAN
GGKYCEGVRV KYRSCNLEPC PSSASGKSFR EEQCEAFNGY NHSTNRLTLA
VAWVPKYSGV SPRDKCKLIC RANGTGYFYV LAPKVVDGTL CSPDSTSVCV
QGKCIKAGCD GNLGSKKRFD KCGVCGGDNK SCKKVTGLFT KPMHGYNFVV
AIPAGASSID IRQRGYKGLI GDDNYLALKN SQGKYLLNGH FVVSAVERDL
VVKGSLLRYS GTGTAVESLQ ASRPILEPLT VEVLSVGKMT PPRVRYSFYL
PKEPREDKSS HPKDPRGPSV LHNSVLSLSN QVEQPDDRPP ARWVAGSWGP
CSASCGSGLQ KRAVDCRGSA GQRTVPACDA AHRPVETQAC GEPCPTWELS
AWSPCSKSCG RGFQRRSLKC VGHGGRLLAR DQCNLHRKPQ ELDFCVLRPC
UCSC uc010scd.3; human.;

Category

Human ADAMTS15 ELISA Kit