Mouse Lcn2 ELISA Kit

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  • Alternative name

    Mouse NGAL ELISA Kit;Mouse Lipocalin-2 ELISA Kit;Mouse SV-40-induced 24P3 protein ELISA Kit;Mouse siderocalin LCN2 ELISA Kit;Mouse p25 ELISA Kit;Mouse 24p3 ELISA Kit;Mouse AW212229 ELISA Kit;Mouse NRL ELISA Kit;Mouse Sip24 ELISA Kit;Mouse lipocalin 2 ELISA Kit;Mouse neutrophil gelatinase-associated lipocalin ELISA Kit;Mouse neu-related lipocalin ELISA Kit;Mouse secreted inducible protein 24 ELISA Kit;

  • Catalog
    E058810
  • species
    Mouse
  • GeneLcn2
  • Standard CurveMouse Lcn2 ELISA Kit
  • Other Species Human LCN2 ELISA KitHuman NGAL/LCN2 ELISA KitMouse NGAL/LCN2 ELISA KitRat Lcn2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LCN2. No significant cross-reactivity or interference between LCN2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LCN2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Lcn2 ELISA Kit allows for the in vitro quantitative determination of Lcn2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: LCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LCN2 antibody and an LCN2-HRP conjugate. The assay sample and buffer are incubated together with LCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCN2 concentration since LCN2 from samples and LCN2-HRP conjugate compete for the anti-LCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LCN2 from the sample, fewer sites are left to bind LCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCN2 concentration in each sample is interpolated from this standard curve.
  • Mouse Neutrophil gelatinase-associated lipocalin Protein information
  • Uniprot ID NGAL_MOUSE
  • Uniprot AC P11672; Q3UE34;
  • UniGene Mm.9537;
  • GeneID 16819
  • KEGG mmu:16819;
  • Mouse Neutrophil gelatinase-associated lipocalin Protein SEQUENCE
  • SEQUENCE 200 AA; 22875 MW; DD9A8D08750E6863 CRC64;

    MALSVMCLGL ALLGVLQSQA QDSTQNLIPA PSLLTVPLQP DFRSDQFRGR

    WYVVGLAGNA VQKKTEGSFT MYSTIYELQE NNSYNVTSIL VRDQDQGCRY

    WIRTFVPSSR AGQFTLGNMH RYPQVQSYNV QVATTDYNQF AMVFFRKTSE

    NKQYFKITLY GRTKELSPEL KERFTRFAKS LGLKDDNIIF SVPTDQCIDN

  • UCSC uc008jfl.1; mouse.;


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