Mouse Lcn2 ELISA Kit

Alternative name
Mouse NGAL ELISA Kit;Mouse Lipocalin-2 ELISA Kit;Mouse SV-40-induced 24P3 protein ELISA Kit;Mouse siderocalin LCN2 ELISA Kit;Mouse p25 ELISA Kit;Mouse 24p3 ELISA Kit;Mouse AW212229 ELISA Kit;Mouse NRL ELISA Kit;Mouse Sip24 ELISA Kit;Mouse lipocalin 2 ELISA Kit;Mouse neutrophil gelatinase-associated lipocalin ELISA Kit;Mouse neu-related lipocalin ELISA Kit;Mouse secreted inducible protein 24 ELISA Kit;
Catalog
E058810
species
Mouse
GeneLcn2
SpecificityThis assay has high sensitivity and excellent specificity for detection of LCN2. No significant cross-reactivity or interference between LCN2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LCN2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Lcn2 ELISA Kit allows for the in vitro quantitative determination of Lcn2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: LCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LCN2 antibody and an LCN2-HRP conjugate. The assay sample and buffer are incubated together with LCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCN2 concentration since LCN2 from samples and LCN2-HRP conjugate compete for the anti-LCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LCN2 from the sample, fewer sites are left to bind LCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCN2 concentration in each sample is interpolated from this standard curve.
Mouse Neutrophil gelatinase-associated lipocalin Protein information
Uniprot ID NGAL_MOUSE
Uniprot AC P11672; Q3UE34;
UniGene Mm.9537;
GeneID 16819
KEGG mmu:16819;
Mouse Neutrophil gelatinase-associated lipocalin Protein SEQUENCE
SEQUENCE 200 AA; 22875 MW; DD9A8D08750E6863 CRC64;
MALSVMCLGL ALLGVLQSQA QDSTQNLIPA PSLLTVPLQP DFRSDQFRGR
WYVVGLAGNA VQKKTEGSFT MYSTIYELQE NNSYNVTSIL VRDQDQGCRY
WIRTFVPSSR AGQFTLGNMH RYPQVQSYNV QVATTDYNQF AMVFFRKTSE
NKQYFKITLY GRTKELSPEL KERFTRFAKS LGLKDDNIIF SVPTDQCIDN
UCSC uc008jfl.1; mouse.;

Mouse Lcn2 ELISA Kit

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