Mouse Lcat ELISA Kit

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  • Alternative name

    Mouse Lecithin-cholesterol acyltransferase ELISA Kit;Mouse phospholipid-cholesterol acyltransferase ELISA Kit;Mouse AI046659 ELISA Kit;Mouse D8Wsu61e ELISA Kit;Mouse lecithin cholesterol acyltransferase ELISA Kit;Mouse phosphatidylcholine-sterol acyltransferase ELISA Kit;Mouse lecithin-cholesterol acyltransferase Lcat ELISA Kit;

  • Catalog
    E058386
  • species
    Mouse
  • GeneLcat
  • Other Species Human LCAT ELISA KitChicken LCAT ELISA KitPorcine LCAT ELISA KitRabbit LCAT ELISA KitRat Lcat ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse LCAT. No significant cross-reactivity or interference between Mouse LCAT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse LCAT and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.375ng/mL
  • Intended UseMouse Lcat ELISA Kit allows for the in vitro quantitative determination of Lcat , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LCAT in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LCAT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse LCAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LCAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse LCAT. You can calculate the concentration of Mouse LCAT in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Phosphatidylcholine-sterol acyltransferase Protein information
  • Uniprot ID LCAT_MOUSE
  • Uniprot AC P16301; Q8K139;
  • UniGene Mm.1593;
  • GeneID 16816
  • KEGG mmu:16816;
  • Mouse Phosphatidylcholine-sterol acyltransferase Protein SEQUENCE
  • SEQUENCE 438 AA; 49747 MW; 2FDD571853523136 CRC64;

    MGLPGSPWQR VLLLLGLLLP PATPFWLLNV LFPPHTTPKA ELSNHTRPVI

    LVPGCLGNRL EAKLDKPDVV NWMCYRKTED FFTIWLDFNL FLPLGVDCWI

    DNTRIVYNHS SGRVSNAPGV QIRVPGFGKT ESVEYVDDNK LAGYLHTLVQ

    NLVNNGYVRD ETVRAAPYDW RLAPHQQDEY YKKLAGLVEE MYAAYGKPVF

    LIGHSLGCLH VLHFLLRQPQ SWKDHFIDGF ISLGAPWGGS IKAMRILASG

    DNQGIPILSN IKLKEEQRIT TTSPWMLPAP HVWPEDHVFI STPNFNYTVQ

    DFERFFTDLH FEEGWHMFLQ SRDLLERLPA PGVEVYCLYG VGRPTPHTYI

    YDHNFPYKDP VAALYEDGDD TVATRSTELC GQWQGRQSQP VHLLPMNETD

    HLNMVFSNKT LEHINAILLG AYRTPKSPAA SPSPPPPE

  • UCSC uc009neq.2; mouse.;


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