Mouse Lcat ELISA Kit

Alternative name
Mouse Lecithin-cholesterol acyltransferase ELISA Kit;Mouse phospholipid-cholesterol acyltransferase ELISA Kit;Mouse AI046659 ELISA Kit;Mouse D8Wsu61e ELISA Kit;Mouse lecithin cholesterol acyltransferase ELISA Kit;Mouse phosphatidylcholine-sterol acyltransferase ELISA Kit;Mouse lecithin-cholesterol acyltransferase Lcat ELISA Kit;
Catalog
E058386
species
Mouse
GeneLcat
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse LCAT. No significant cross-reactivity or interference between Mouse LCAT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse LCAT and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.375ng/mL
Intended UseMouse Lcat ELISA Kit allows for the in vitro quantitative determination of Lcat , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LCAT in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LCAT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse LCAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LCAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse LCAT. You can calculate the concentration of Mouse LCAT in the samples by comparing the OD of the samples to the standard curve.
Mouse Phosphatidylcholine-sterol acyltransferase Protein information
Uniprot ID LCAT_MOUSE
Uniprot AC P16301; Q8K139;
UniGene Mm.1593;
GeneID 16816
KEGG mmu:16816;
Mouse Phosphatidylcholine-sterol acyltransferase Protein SEQUENCE
SEQUENCE 438 AA; 49747 MW; 2FDD571853523136 CRC64;
MGLPGSPWQR VLLLLGLLLP PATPFWLLNV LFPPHTTPKA ELSNHTRPVI
LVPGCLGNRL EAKLDKPDVV NWMCYRKTED FFTIWLDFNL FLPLGVDCWI
DNTRIVYNHS SGRVSNAPGV QIRVPGFGKT ESVEYVDDNK LAGYLHTLVQ
NLVNNGYVRD ETVRAAPYDW RLAPHQQDEY YKKLAGLVEE MYAAYGKPVF
LIGHSLGCLH VLHFLLRQPQ SWKDHFIDGF ISLGAPWGGS IKAMRILASG
DNQGIPILSN IKLKEEQRIT TTSPWMLPAP HVWPEDHVFI STPNFNYTVQ
DFERFFTDLH FEEGWHMFLQ SRDLLERLPA PGVEVYCLYG VGRPTPHTYI
YDHNFPYKDP VAALYEDGDD TVATRSTELC GQWQGRQSQP VHLLPMNETD
HLNMVFSNKT LEHINAILLG AYRTPKSPAA SPSPPPPE
UCSC uc009neq.2; mouse.;

Category

Mouse Lcat ELISA Kit