Mouse Lamc2 ELISA Kit

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  • Alternative name

    Mouse Epiligrin subunit gamma ELISA Kit;Mouse Kalinin subunit gamma ELISA Kit;Mouse Kalinin/nicein/epiligrin 100 kDa subunit ELISA Kit;Mouse Laminin B2t chain ELISA Kit;Mouse Laminin-5 subunit gamma ELISA Kit;Mouse Nicein subunit gamma ELISA Kit;

  • Catalog
    E058306
  • species
    Mouse
  • GeneLamc2
  • Standard CurveMouse Lamc2 ELISA Kit
  • Other Species Human LAMC2 ELISA KitHorse LAMC2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10 pg/ml
  • Intended UseMouse Lamc2 ELISA Kit allows for the in vitro quantitative determination of Lamc2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Laminin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Laminin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Laminin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Laminin can be calculated. Background/Introduction: Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. Fifteen laminin trimers have been identified. The laminins are combinations of different alpha-, beta-, and gamma-chains. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Recently, several publications have demonstrated that laminins can be used to culture cells, such as pluripotent stem cells, that are difficult to culture on other substrates. Rodin et al. showed that recombinant laminin 511 can be used to create a totally xeno-free and defined cell culture environment to culture human pluripotent ES cells and human iPS cells.
  • Mouse Laminin subunit gamma-2 Protein information
  • Uniprot ID LAMC2_MOUSE
  • Uniprot AC Q61092;
  • UniGene Mm.329272;
  • Mouse Laminin subunit gamma-2 Protein SEQUENCE
  • SEQUENCE 1191 AA; 130161 MW; 7016C1F851D909B9 CRC64;

    MPALWLSCCL GVALLLPAAQ ATSRREVCDC NGKSRQCVFD QELHRQTGSG

    FRCLNCNDNT AGVHCERCRE GFYRHRDRDR CLPCNCHSKG SLSAGCDNSG

    QCRCKPGVTG QRCDRCQPGF HMLTDAGCTR DQGQLDSKCD CDPAGISGPC

    DSGRCVCKPA VTGERCDRCR PGYYHLDRAN PEGCTQCFCY GHSASCHASA

    DFSVHKITST FSQDVDGWKA VQRNGAPAKL HWSQRHRDVF SSARRSDPVY

    FVAPAKFLGN QQVSYGQSLS FDYRVDRGGR QPSAYDVILE GAGLQIRAPL

    MAPGKTLPCG ITKTYTFRLN EHPSSHWSPQ LSYFEYRRLL RNLTALLIRA

    TYGEYSTGYI DNVTLVSARP VSGAPAPWVE RCVCPAGYKG QFCQECASGY

    KRDSARLGPF GACVPCNCQG GGACDPDTGD CYSGDENPDI ECADCPIGFY

    NDPHDPRSCK PCPCHNGFSC SVMPETEEVV CNNCPPGVTG ARCELCADGF

    FGDPFGERGP VRPCQRCQCN NNVDPNASGN CDQLTGRCLK CIYNTAGVYC

    DQCKAGYFGD PLAPNPADKC RACNCSPMGS EPGECRGDGS CVCKPGFGGL

    NCDHAALTSC PACYNQVKIQ MDQFTQQLQS LEALVSKAQG GGGGGTVPVQ

    LEGRIEQAEQ ALQDILGEAQ ISEGAMRAVA VRLAKARSQE NDYKTRLDDL

    KMTAERIRAL GSQHQNRVQD TSRLISQMRL SLAGSEALLE NTNIHSSEHY

    VGPNDFKSLA QEATRKADSH AESANAMKQL ARETEDYSKQ ALSLARKLLS

    GGGGSGSWDS SVVQGLMGKL EKTKSLSQQL SLEGTQADIE ADRSYQHSLR

    LLDSASQLQG VSDLSFQVEA KRIRQKADSL SNLVTRQTDA FTRVRNNLGN

    WEKETRQLLQ TGKDRRQTSD QLLSRANLAK NRAQEALSMG NATFYEVENI

    LKNLREFDLQ VEDRKAEAEE AMKRLSSISQ KVADASDKTQ QAETALGSAT

    ADTQRAKNAA REALEISSEI ELEIGSLNLE ANVTADGALA MEKGTATLKS

    EMREMIELAR KELEFDTDKD TVQLVITEAQ QADARATSAG VTIQDTLNTL

    DGILHLIDQP GSVDEEGMML LEQGLFQAKT QINSRLRPLM SDLEERVRRQ

    RNHLHLLETS IDGILADVKN LENIRDNLPP GCYNTQALEQ Q



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