Mouse Lamc2 ELISA Kit

Alternative name
Mouse Epiligrin subunit gamma ELISA Kit;Mouse Kalinin subunit gamma ELISA Kit;Mouse Kalinin/nicein/epiligrin 100 kDa subunit ELISA Kit;Mouse Laminin B2t chain ELISA Kit;Mouse Laminin-5 subunit gamma ELISA Kit;Mouse Nicein subunit gamma ELISA Kit;
Catalog
E058306
species
Mouse
GeneLamc2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10 pg/ml
Intended UseMouse Lamc2 ELISA Kit allows for the in vitro quantitative determination of Lamc2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Laminin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Laminin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Laminin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Laminin can be calculated. Background/Introduction: Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. Fifteen laminin trimers have been identified. The laminins are combinations of different alpha-, beta-, and gamma-chains. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Recently, several publications have demonstrated that laminins can be used to culture cells, such as pluripotent stem cells, that are difficult to culture on other substrates. Rodin et al. showed that recombinant laminin 511 can be used to create a totally xeno-free and defined cell culture environment to culture human pluripotent ES cells and human iPS cells.
Mouse Laminin subunit gamma-2 Protein information
Uniprot ID LAMC2_MOUSE
Uniprot AC Q61092;
UniGene Mm.329272;
Mouse Laminin subunit gamma-2 Protein SEQUENCE
SEQUENCE 1191 AA; 130161 MW; 7016C1F851D909B9 CRC64;
MPALWLSCCL GVALLLPAAQ ATSRREVCDC NGKSRQCVFD QELHRQTGSG
FRCLNCNDNT AGVHCERCRE GFYRHRDRDR CLPCNCHSKG SLSAGCDNSG
QCRCKPGVTG QRCDRCQPGF HMLTDAGCTR DQGQLDSKCD CDPAGISGPC
DSGRCVCKPA VTGERCDRCR PGYYHLDRAN PEGCTQCFCY GHSASCHASA
DFSVHKITST FSQDVDGWKA VQRNGAPAKL HWSQRHRDVF SSARRSDPVY
FVAPAKFLGN QQVSYGQSLS FDYRVDRGGR QPSAYDVILE GAGLQIRAPL
MAPGKTLPCG ITKTYTFRLN EHPSSHWSPQ LSYFEYRRLL RNLTALLIRA
TYGEYSTGYI DNVTLVSARP VSGAPAPWVE RCVCPAGYKG QFCQECASGY
KRDSARLGPF GACVPCNCQG GGACDPDTGD CYSGDENPDI ECADCPIGFY
NDPHDPRSCK PCPCHNGFSC SVMPETEEVV CNNCPPGVTG ARCELCADGF
FGDPFGERGP VRPCQRCQCN NNVDPNASGN CDQLTGRCLK CIYNTAGVYC
DQCKAGYFGD PLAPNPADKC RACNCSPMGS EPGECRGDGS CVCKPGFGGL
NCDHAALTSC PACYNQVKIQ MDQFTQQLQS LEALVSKAQG GGGGGTVPVQ
LEGRIEQAEQ ALQDILGEAQ ISEGAMRAVA VRLAKARSQE NDYKTRLDDL
KMTAERIRAL GSQHQNRVQD TSRLISQMRL SLAGSEALLE NTNIHSSEHY
VGPNDFKSLA QEATRKADSH AESANAMKQL ARETEDYSKQ ALSLARKLLS
GGGGSGSWDS SVVQGLMGKL EKTKSLSQQL SLEGTQADIE ADRSYQHSLR
LLDSASQLQG VSDLSFQVEA KRIRQKADSL SNLVTRQTDA FTRVRNNLGN
WEKETRQLLQ TGKDRRQTSD QLLSRANLAK NRAQEALSMG NATFYEVENI
LKNLREFDLQ VEDRKAEAEE AMKRLSSISQ KVADASDKTQ QAETALGSAT
ADTQRAKNAA REALEISSEI ELEIGSLNLE ANVTADGALA MEKGTATLKS
EMREMIELAR KELEFDTDKD TVQLVITEAQ QADARATSAG VTIQDTLNTL
DGILHLIDQP GSVDEEGMML LEQGLFQAKT QINSRLRPLM SDLEERVRRQ
RNHLHLLETS IDGILADVKN LENIRDNLPP GCYNTQALEQ Q

Category

Mouse Lamc2 ELISA Kit

Mouse Epiligrin subunit gamma ELISA Kit;Mouse Kalinin subunit gamma ELISA Kit;Mouse Kalinin/nicein/epiligrin 100 kDa subunit ELISA Kit;Mouse Laminin B2t chain ELISA Kit;Mouse Laminin-5 subunit gamma ELISA Kit;Mouse Nicein subunit gamma ELISA Kit;