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Alternative name
L-lactate dehydrogenase 1 ELISA KIT; ldh1 ELISA KIT; ldh ELISA KIT; lmo0210 ELISA KIT; L-LDH 1 ELISA KIT
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Catalog
E058237
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species
Mouse
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GeneLDH
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Standard Curve
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Other Species
Human D LDH ELISA KitHuman D-LDH ELISA KitHuman LDH ELISA KitHuman L LDH ELISA KitHuman LDH-2 ELISA KitHuman LDH-3 ELISA KitHuman LDH-4 ELISA KitMouse D-LDH ELISA KitMouse L-LDH ELISA KitMouse LDH-1 ELISA KitMouse LDH-2 ELISA KitMouse LDH-3 ELISA KitMouse LDH-4 ELISA KitMouse LDH-5 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of LDH. No significant cross-reactivity or interference between LDH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LDH and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL.
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Intended UseMouse LDH ELISA Kit allows for the in vitro quantitative determination of LDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This LDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LDH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||LDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LDH antibody and an LDH-HRP conjugate. The assay sample and buffer are incubated together with LDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LDH concentration since LDH from samples and LDH-HRP conjugate compete for the anti-LDH antibody binding site. Since the number of sites is limited, as more sites are occupied by LDH from the sample, fewer sites are left to bind LDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDH concentration in each sample is interpolated from this standard curve.
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