Mouse Klf15 ELISA Kit

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  • Alternative name

    Mouse cardiovascular Krueppel-like factor ELISA Kit;Mouse CKLF ELISA Kit;Mouse 1810013I09Rik ELISA Kit;Mouse AV048136 ELISA Kit;Mouse AW494632 ELISA Kit;Mouse KKLF ELISA Kit;Mouse Kruppel-like factor 15 ELISA Kit;Mouse Krueppel-like factor 15 ELISA Kit;

  • Catalog
    E058147
  • species
    Mouse
  • GeneKlf15
  • Standard CurveMouse Klf15 ELISA Kit
  • Other Species Human KLF15 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Klf15 ELISA Kit allows for the in vitro quantitative determination of Klf15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the Assay: KLF15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLF15 antibody and an KLF15-HRP conjugate. The assay sample and buffer are incubated together with KLF15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF15 concentration since KLF15 from samples and KLF15-HRP conjugate compete for the anti-KLF15 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF15 from the sample, fewer sites are left to bind KLF15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF15 concentration in each sample is interpolated from this standard curve.
  • Mouse Krueppel-like factor 15 Protein information
  • Uniprot ID KLF15_MOUSE
  • Uniprot AC Q9EPW2; Q3UQB0;
  • UniGene Mm.38343; Mm.41389;
  • GeneID 66277
  • KEGG mmu:66277;
  • Mouse Krueppel-like factor 15 Protein SEQUENCE
  • SEQUENCE 415 AA; 44253 MW; 127A7B80DB3E33CC CRC64;

    MVDHLLPVDE TFSSPKCSVG YLGDRLASRQ PYHMLPSPIS EDDSDVSSPC

    SCASPDSQAF CSCYSAGPGP EAQGSILDFL LSRATLGSGG GSGGIGDSSG

    PVTWGSWRRA SVPVKEEHFC FPEFLSGDTD DVSRPFQPTL EEIEEFLEEN

    MEAEVKEAPE NGSRDLETCS QLSAGSHRSH LHPESAGRER CTPPPGGTSG

    GGAQSAGEGP AHDGPVPVLL QIQPVAVKQE AGTGPASPGQ APESVKVAQL

    LVNIQGQTFA LLPQVVPSSN LNLPSKFVRI APVPIAAKPI GSGSLGPGPA

    GLLVGQKFPK NPAAELLKMH KCTFPGCSKM YTKSSHLKAH LRRHTGEKPF

    ACTWPGCGWR FSRSDELSRH RRSHSGVKPY QCPVCEKKFA RSDHLSKHIK

    VHRFPRSSRA VRAIN

  • UCSC uc009cxj.1; mouse.;


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