Mouse Kl ELISA Kit

Alternative name
Mouse alpha-kl ELISA Kit;Mouse klotho ELISA Kit;Mouse secreted form of Klotho protein ELISA Kit;
Catalog
E058112
species
Mouse
GeneKl
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.4pg/ml
Intended UseMouse Kl ELISA Kit allows for the in vitro quantitative determination of Kl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Kl antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Kl antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Kl amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Kl can be calculated.
Mouse Klotho peptide Protein information
Uniprot ID KLOT_MOUSE
Uniprot AC O35082; B2RR78; O70175; O70621;
UniGene Mm.6500;
GeneID 16591
KEGG mmu:16591;
Mouse Klotho peptide Protein SEQUENCE
SEQUENCE 1014 AA; 116398 MW; 24BD772A1F81B8EC CRC64;
MLARAPPRRP PRLVLLRLLL LHLLLLALRA RCLSAEPGQG AQTWARFARA
PAPEAAGLLH DTFPDGFLWA VGSAAYQTEG GWRQHGKGAS IWDTFTHHSG
AAPSDSPIVV APSGAPSPPL SSTGDVASDS YNNVYRDTEG LRELGVTHYR
FSISWARVLP NGTAGTPNRE GLRYYRRLLE RLRELGVQPV VTLYHWDLPQ
RLQDTYGGWA NRALADHFRD YAELCFRHFG GQVKYWITID NPYVVAWHGY
ATGRLAPGVR GSSRLGYLVA HNLLLAHAKV WHLYNTSFRP TQGGRVSIAL
SSHWINPRRM TDYNIRECQK SLDFVLGWFA KPIFIDGDYP ESMKNNLSSL
LPDFTESEKR LIRGTADFFA LSFGPTLSFQ LLDPNMKFRQ LESPNLRQLL
SWIDLEYNHP PIFIVENGWF VSGTTKRDDA KYMYYLKKFI METLKAIRLD
GVDVIGYTAW SLMDGFEWHR GYSIRRGLFY VDFLSQDKEL LPKSSALFYQ
KLIEDNGFPP LPENQPLEGT FPCDFAWGVV DNYVQVDTTL SQFTDPNVYL
WDVHHSKRLI KVDGVVAKKR KPYCVDFSAI RPQITLLREM RVTHFRFSLD
WALILPLGNQ TQVNHTVLHF YRCMISELVH ANITPVVALW QPAAPHQGLP
HALAKHGAWE NPHTALAFAD YANLCFKELG HWVNLWITMN EPNTRNMTYR
AGHHLLRAHA LAWHLYDDKF RAAQKGKISI ALQADWIEPA CPFSQNDKEV
AERVLEFDIG WLAEPIFGSG DYPRVMRDWL NQKNNFLLPY FTEDEKKLVR
GSFDFLAVSH YTTILVDWEK EDPMKYNDYL EVQEMTDITW LNSPSQVAVV
PWGLRKVLNW LRFKYGDLPM YVTANGIDDD PHAEQDSLRI YYIKNYVNEA
LKAYVLDDIN LCGYFAYSLS DRSAPKSGFY RYAANQFEPK PSMKHYRKII
DSNGFLGSGT LGRFCPEEYT VCTECGFFQT RKSLLVFISF LVFTFIISLA
LIFHYSKKGQ RSYK
UCSC uc009aul.2; mouse. [O35082-1];

Category

Mouse Kl ELISA Kit

Mouse alpha-kl ELISA Kit;Mouse klotho ELISA Kit;Mouse secreted form of Klotho protein ELISA Kit;