SpecificityCA II and CA XII are two related members of the carbonic anhydrase family. The CA IX ELISA was challenged with high levels of each analyte. Both enzymes showed less than 5% cross-reactivity with CA IX.
SensitivityA minimal detectable concentration of analyte was determined by repeated measurement of a zero dose sample (Standard Level 1) and calculation of mean + 2 standard deviations. The CA IX ELISA will detect 5.0 pg/mL of CA IX when read off a curve using the v
Intended UseHuman CA IX ELISA Kit allows for the in vitro quantitative determination of CA IX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyOncology; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: The CA IX ELISA is a sandwich-type immunoassay that uses a mouse monoclonal Capture Antibody (V10) and a biotinylated mouse monoclonal antibody (M75) as detector . The Capture Antibody has been immobilized on the interior surface of the microtiter plate wells. To perform the test, an appropriate volume of specimen is incubated in the wells to allow binding of the antigen by the Capture Antibody. The immobilized antigen is then exposed to the biotinylated Detector Antibody. A streptavidin-HRP Conjugate is then added. Addition of Substrate to the wells allows the catalysis of a chromogen into a colored product, the intensity of which is proportional to the amount of CA IX protein, also known as MN, that is bound to the plate. Standards are provided in the kit that allow accurate, quantitative determinations of CA IX in suitable samples. Using a microtiter plate reader, one can measure simultaneously the absorbance of the colored product in the Standards and sample wells. Correlating the absorbance values of samples with the Standards allows the investigator to determine the levels of CA IX in a sample. Samples may be assigned a quantitative value of CA IX in picograms per mL (pg/mL) of serum and plasma. For instructions, see the Detailed Protocol and Evaluation of Results sections of this booklet.