Human CA 72-4 ELISA Kit

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  • Catalog
  • species
  • GeneCA 72-4
  • SpecificityNo cross reactivity was observed with related proteins.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe analytical sensitivity was calculated by adding 2 standard deviations to the mean of 20 replicate analyses of Standard 0 and was found to be 0.79 U/mL.
  • Intended UseHuman CA 72-4 ELISA Kit allows for the in vitro quantitative determination of CA 72-4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyOncology; Research Assays; Assay Categories
  • Product Description
    Principle of the assay: The Biobool CA 72-4 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal mouse antibody (Clone CC49) directed towards a unique antigenic site on a CA 72-4 molecule. An aliquot of sample containing endogenous CA 72-4 is incubated in the coated well with enzyme conjugate, which is an anti-CA 72-4 antibody (Clone B72.3) conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 72-4 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 72-4 in the sample.

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