Human C8B ELISA Kit

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  • Alternative name

    Human complement component 8 subunit beta ELISA Kit;Human C82 ELISA Kit;Human complement C8 beta chain ELISA Kit;Human complement component C8 beta chain ELISA Kit;Human complement component 8, beta polypeptide ELISA Kit;

  • Catalog
    E005786
  • species
    Human
  • GeneC8B
  • Other Species Mouse C8b ELISA KitRabbit C8B ELISA KitRat C8b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human C8b. No significant cross-reactivity or interference between Human C8b and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human C8b and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman C8B ELISA Kit allows for the in vitro quantitative determination of C8B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C8b in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C8b. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human C8b and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C8b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C8b. You can calculate the concentration of Human C8b in the samples by comparing the OD of the samples to the standard curve.
  • Human Complement component C8 beta chain Protein information
  • Uniprot ID CO8B_HUMAN
  • Uniprot AC P07358; A1L4K7;
  • UniGene Hs.391835;
  • GeneID 732
  • KEGG hsa:732;
  • Human Complement component C8 beta chain Protein SEQUENCE
  • SEQUENCE 591 AA; 67047 MW; B01722A6F2E9AFCE CRC64;

    MKNSRTWAWR APVELFLLCA ALGCLSLPGS RGERPHSFGS NAVNKSFAKS

    RQMRSVDVTL MPIDCELSSW SSWTTCDPCQ KKRYRYAYLL QPSQFHGEPC

    NFSDKEVEDC VTNRPCRSQV RCEGFVCAQT GRCVNRRLLC NGDNDCGDQS

    DEANCRRIYK KCQHEMDQYW GIGSLASGIN LFTNSFEGPV LDHRYYAGGC

    SPHYILNTRF RKPYNVESYT PQTQGKYEFI LKEYESYSDF ERNVTEKMAS

    KSGFSFGFKI PGIFELGISS QSDRGKHYIR RTKRFSHTKS VFLHARSDLE

    VAHYKLKPRS LMLHYEFLQR VKRLPLEYSY GEYRDLFRDF GTHYITEAVL

    GGIYEYTLVM NKEAMERGDY TLNNVHACAK NDFKIGGAIE EVYVSLGVSV

    GKCRGILNEI KDRNKRDTMV EDLVVLVRGG ASEHITTLAY QELPTADLMQ

    EWGDAVQYNP AIIKVKVEPL YELVTATDFA YSSTVRQNMK QALEEFQKEV

    SSCHCAPCQG NGVPVLKGSR CDCICPVGSQ GLACEVSYRK NTPIDGKWNC

    WSNWSSCSGR RKTRQRQCNN PPPQNGGSPC SGPASETLDC S

  • UCSC uc001cyp.5; human.;




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