SpecificityThis assay has high sensitivity and excellent specificity for detection of KEAP1. No significant cross-reactivity or interference between KEAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KEAP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse KEAP1 ELISA Kit allows for the in vitro quantitative determination of KEAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description specificalPrinciple of the assay: KEAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KEAP1 antibody and an KEAP1-HRP conjugate. The assay sample and buffer are incubated together with KEAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KEAP1 concentration since KEAP1 from samples and KEAP1-HRP conjugate compete for the anti-KEAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KEAP1 from the sample, fewer sites are left to bind KEAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KEAP1 concentration in each sample is interpolated from this standard curve.