Human C5AR1 ELISA Kit

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  • Alternative name

    Human C5a anaphylatoxin chemotactic receptor ELISA Kit;Human C5a-R ELISA Kit;Human C5AR ELISA Kit;Human C5R1 ELISA Kit;Human C5A ELISA Kit;Human CD88 ELISA Kit;Human complement C5a receptor 1 ELISA Kit;Human C5a anaphylatoxin chemotactic receptor 1 ELISA Kit;Human C5a anaphylatoxin receptor ELISA Kit;Human C5a ligand ELISA Kit;Human complement component 5 receptor 1 ELISA Kit;Human complement component 5a receptor 1 ELISA Kit;

  • Catalog
    E005780
  • species
    Human
  • GeneC5AR1
  • Standard CurveHuman C5AR1 ELISA Kit
  • Other Species Mouse C5ar1 ELISA KitCanine C5AR1 ELISA KitGuinea Pig C5AR1 ELISA KitRabbit C5AR1 ELISA KitRat C5ar1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of C5AR1. No significant cross-reactivity or interference between C5AR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C5AR1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman C5AR1 ELISA Kit allows for the in vitro quantitative determination of C5AR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This C5AR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C5AR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||C5AR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C5AR1 antibody and an C5AR1-HRP conjugate. The assay sample and buffer are incubated together with C5AR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C5AR1 concentration since C5AR1 from samples and C5AR1-HRP conjugate compete for the anti-C5AR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by C5AR1 from the sample, fewer sites are left to bind C5AR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5AR1 concentration in each sample is interpolated from this standard curve.
  • Human C5a anaphylatoxin chemotactic receptor 1 Protein information
  • Uniprot ID C5AR1_HUMAN
  • Uniprot AC P21730;
  • UniGene Hs.2161;
  • GeneID 728
  • KEGG hsa:728;
  • Human C5a anaphylatoxin chemotactic receptor 1 Protein SEQUENCE
  • SEQUENCE 350 AA; 39336 MW; 9334BB39A2C96D3D CRC64;

    MDSFNYTTPD YGHYDDKDTL DLNTPVDKTS NTLRVPDILA LVIFAVVFLV

    GVLGNALVVW VTAFEAKRTI NAIWFLNLAV ADFLSCLALP ILFTSIVQHH

    HWPFGGAACS ILPSLILLNM YASILLLATI SADRFLLVFK PIWCQNFRGA

    GLAWIACAVA WGLALLLTIP SFLYRVVREE YFPPKVLCGV DYSHDKRRER

    AVAIVRLVLG FLWPLLTLTI CYTFILLRTW SRRATRSTKT LKVVVAVVAS

    FFIFWLPYQV TGIMMSFLEP SSPTFLLLKK LDSLCVSFAY INCCINPIIY

    VVAGQGFQGR LRKSLPSLLR NVLTEESVVR ESKSFTRSTV DTMAQKTQAV

  • UCSC uc002pgj.2; human.;




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