Human C4BPA ELISA Kit

Alternative name
Human C4BP ELISA Kit;Human proline-rich protein ELISA Kit;Human PRP ELISA Kit;Human complement component 4 binding protein alpha ELISA Kit;Human C4b-binding protein alpha chain ELISA Kit;
Catalog
E005759
species
Human
GeneC4BPA
Standard Curve
Other Species Mouse C4bpa ELISA Kit Bovine C4BPA ELISA Kit Rat C4bpa ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of C4 Binding Protein Alpha (C4BPa). No significant cross-reactivity or interference between C4 Binding Protein Alpha (C4BPa) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.068ng/mL.
Intended UseHuman C4BPA ELISA Kit allows for the in vitro quantitative determination of C4BPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4 Binding Protein Alpha (C4BPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C4 Binding Protein Alpha (C4BPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4 Binding Protein Alpha (C4BPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4 Binding Protein Alpha (C4BPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human C4b-binding protein alpha chain Protein information
Uniprot ID C4BPA_HUMAN
Uniprot AC P04003; Q5VVQ8;
UniGene Hs.1012;
GeneID 722
KEGG hsa:722;
Human C4b-binding protein alpha chain Protein SEQUENCE
SEQUENCE 597 AA; 67033 MW; 67E03F2EA85A16DD CRC64;
MHPPKTPSGA LHRKRKMAAW PFSRLWKVSD PILFQMTLIA ALLPAVLGNC
GPPPTLSFAA PMDITLTETR FKTGTTLKYT CLPGYVRSHS TQTLTCNSDG
EWVYNTFCIY KRCRHPGELR NGQVEIKTDL SFGSQIEFSC SEGFFLIGST
TSRCEVQDRG VGWSHPLPQC EIVKCKPPPD IRNGRHSGEE NFYAYGFSVT
YSCDPRFSLL GHASISCTVE NETIGVWRPS PPTCEKITCR KPDVSHGEMV
SGFGPIYNYK DTIVFKCQKG FVLRGSSVIH CDADSKWNPS PPACEPNSCI
NLPDIPHASW ETYPRPTKED VYVVGTVLRY RCHPGYKPTT DEPTTVICQK
NLRWTPYQGC EALCCPEPKL NNGEITQHRK SRPANHCVYF YGDEISFSCH
ETSRFSAICQ GDGTWSPRTP SCGDICNFPP KIAHGHYKQS SSYSFFKEEI
IYECDKGYIL VGQAKLSCSY SHWSAPAPQC KALCRKPELV NGRLSVDKDQ
YVEPENVTIQ CDSGYGVVGP QSITCSGNRT WYPEVPKCEW ETPEGCEQVL
TGKRLMQCLP NPEDVKMALE VYKLSLEIEQ LELQRDSARQ STLDKEL
UCSC uc001hfo.3; human.;

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Category

Human C4BPA ELISA Kit

Human C4BP ELISA Kit;Human proline-rich protein ELISA Kit;Human PRP ELISA Kit;Human complement component 4 binding protein alpha ELISA Kit;Human C4b-binding protein alpha chain ELISA Kit;