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Mouse IL6R ELISA Kit

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  • Alternative name

    Interleukin-6 receptor subunit alpha ELISA KIT; IL-6R 1 ELISA KIT; Membrane glycoprotein 80 ELISA KIT; gp80 ELISA KIT; CD_antigen: CD126IL6R ELISA KIT; IL-6 receptor subunit alpha ELISA KIT; IL-6R subunit alpha ELISA KIT; IL-6R-alpha ELISA KIT; IL-6RA ELISA KIT; gp80 ELISA KIT
  • Catalog
    E057394
  • species
    Mouse
  • GeneIL6R
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 6 Receptor (IL6R). No significant cross-reactivity or interference between Interleukin 6 Receptor (IL6R) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.148ng/mL.
  • Intended UseMouse IL6R ELISA Kit allows for the in vitro quantitative determination of IL6R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 Receptor (IL6R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 Receptor (IL6R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 Receptor (IL6R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 Receptor (IL6R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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