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Mouse Il5ra ELISA Kit

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  • Alternative name

    Mouse IL-5 receptor subunit alpha ELISA Kit;Mouse IL-5R subunit alpha ELISA Kit;Mouse IL-5R-alpha ELISA Kit;Mouse IL-5RA ELISA Kit;Mouse Il5r ELISA Kit;Mouse CD125 ELISA Kit;Mouse CDw125 ELISA Kit;Mouse interleukin 5 receptor, alpha ELISA Kit;Mouse interleukin-5 receptor subunit alpha ELISA Kit;Mouse IL-5 receptor alpha chain ELISA Kit;
  • Catalog
    E057384
  • species
    Mouse
  • GeneIl5ra
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 5 Receptor Alpha (IL5Ra). No significant cross-reactivity or interference between Interleukin 5 Receptor Alpha (IL5Ra) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 28.5pg/mL.
  • Intended UseMouse Il5ra ELISA Kit allows for the in vitro quantitative determination of Il5ra , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha (IL5Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 5 Receptor Alpha (IL5Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 Receptor Alpha (IL5Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 5 Receptor Alpha (IL5Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Interleukin-5 receptor subunit alpha Protein information
  • Uniprot ID IL5RA_MOUSE
  • Uniprot AC P21183;
  • UniGene Mm.3448;
  • GeneID 16192
  • KEGG mmu:16192;
  • Mouse Interleukin-5 receptor subunit alpha Protein SEQUENCE

  • SEQUENCE 415 AA; 46989 MW; A4326D2922571C08 CRC64;

    MVPVLLILVG ALATLQADLL NHKKFLLLPP VNFTIKATGL AQVLLHWDPN

    PDQEQRHVDL EYHVKINAPQ EDEYDTRKTE SKCVTPLHEG FAASVRTILK

    SSHTTLASSW VSAELKAPPG SPGTSVTNLT CTTHTVVSSH THLRPYQVSL

    RCTWLVGKDA PEDTQYFLYY RFGVLTEKCQ EYSRDALNRN TACWFPRTFI

    NSKGFEQLAV HINGSSKRAA IKPFDQLFSP LAIDQVNPPR NVTVEIESNS

    LYIQWEKPLS AFPDHCFNYE LKIYNTKNGH IQKEKLIANK FISKIDDVST

    YSIQVRAAVS SPCRMPGRWG EWSQPIYVGK ERKSLVEWHL IVLPTAACFV

    LLIFSLICRV CHLWTRLFPP VPAPKSNIKD LPVVTEYEKP SNETKIEVVH

    CVEEVGFEVM GNSTF

  • UCSC uc009dcx.1; mouse.;



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