Mouse INS ELISA Kit

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  • Alternative name

    Insulin ELISA KIT; INS ELISA KIT;

  • Catalog
    E056780
  • species
    Mouse
  • GeneINS
  • Other Species Human INS ELISA KitHuman INS-IGF2 ELISA KitBovine INS ELISA KitCanine INS ELISA KitGoat INS ELISA KitGuinea Pig INS ELISA KitChicken INS ELISA KitHorse INS ELISA KitMonkey INS ELISA KitTurkey INS ELISA KitPorcine INS ELISA KitRabbit INS ELISA KitSheep INS ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity3.9 nIU/ml
  • Intended UseMouse INS ELISA Kit allows for the in vitro quantitative determination of INS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to INS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for INS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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