Intended UseMouse Insl3 ELISA Kit allows for the in vitro quantitative determination of Insl3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalBackground/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated INSL3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse INSL3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add INSL3 and INSL3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INSL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.