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Catalog
E056457
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species
Mouse
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GeneIgG1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Immunoglobulin G1 (IgG1).
No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 259.3ng/mL.
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Intended UseMouse IgG1 ELISA Kit allows for the in vitro quantitative determination of IgG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin G1 (IgG1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Immunoglobulin G1 (IgG1) and unlabeled Immunoglobulin G1 (IgG1) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G1 (IgG1). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample.
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