Mouse IgD ELISA Kit

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  • Catalog
    E056434
  • species
    Mouse
  • GeneIgD
  • Standard CurveMouse IgD ELISA Kit
  • Other Species Human HAV IgD ELISA KitHuman HDV IgD ELISA KitHuman IgD ELISA KitHuman IM IgD ELISA KitHuman Tox IgD ELISA KitMouse HAV IgD ELISA KitMouse HDV IgD ELISA KitMouse IM IgD ELISA KitMouse RSV-IgD ELISA KitMouse Tox IgD ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse IgD ELISA Kit allows for the in vitro quantitative determination of IgD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Intended Uses: This IgD ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IgDin the sample, this IgDELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IgDconcentration. The concentration of IgDin the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This IgDenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IgD. Standards or samples are then added to the microtiter plate wells and IgDif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgDpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgDare added to each well to "sandwich" the IgDimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain IgDand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.



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