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Alternative name
CMRF35-like molecule 1 ELISA KIT; CD300 antigen-like family member F ELISA KIT; Immune receptor expressed on myeloid cells 1 ELISA KIT; IREM-1 ELISA KIT; Immunoglobulin superfamily member 13 ELISA KIT; IgSF13 ELISA KIT; NK inhibitory receptor ELISA KIT; CD_antigen: CD300fCD300LF ELISA KIT; CD300F ELISA KIT; CLM1 ELISA KIT; IGSF13 ELISA KIT; IREM1 ELISA KIT; NKIR ELISA KIT; CLM-1 ELISA KIT; IREM-1 ELISA KIT; IgSF13 ELISA KIT
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Catalog
E056415
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species
Mouse
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GeneIREM1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Immune Receptor Expressed On Myeloid Cells 1 (IREM1).
No significant cross-reactivity or interference between Immune Receptor Expressed On Myeloid Cells 1 (IREM1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.62ng/mL.
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Intended UseMouse IREM1 ELISA Kit allows for the in vitro quantitative determination of IREM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immune Receptor Expressed On Myeloid Cells 1 (IREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immune Receptor Expressed On Myeloid Cells 1 (IREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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