Mouse Il7 ELISA Kit

Alternative name
Mouse Il-7 ELISA Kit;Mouse A630026I06Rik ELISA Kit;Mouse hlb368 ELISA Kit;Mouse interleukin 7 ELISA Kit;Mouse interleukin-7 ELISA Kit;
Catalog
E056393
species
Mouse
GeneIl7
SpecificityNatural and recombinant mouse IL-7
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseMouse Il7 ELISA Kit allows for the in vitro quantitative determination of Il7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli,E26-I154) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-7 amount of sample captured in plate. Background: Interleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T(H)17) cells inexperimental autoimmune encephalomyelitis (EAE).2
Mouse Interleukin-7 Protein information
Uniprot ID IL7_MOUSE
Uniprot AC P10168;
UniGene Mm.3825;
GeneID 16196
KEGG mmu:16196;
Mouse Interleukin-7 Protein SEQUENCE
SEQUENCE 154 AA; 17727 MW; CBAAB7A1A7516A42 CRC64;
MFHVSFRYIF GIPPLILVLL PVTSSECHIK DKEGKAYESV LMISIDELDK
MTGTDSNCPN NEPNFFRKHV CDDTKEAAFL NRAARKLKQF LKMNISEEFN
VHLLTVSQGT QTLVNCTSKE EKNVKEQKKN DACFLKRLLR EIKTCWNKIL
KGSI
UCSC uc008ooi.1; mouse.;

Mouse Il7 ELISA Kit

Mouse Il-7 ELISA Kit;Mouse A630026I06Rik ELISA Kit;Mouse hlb368 ELISA Kit;Mouse interleukin 7 ELISA Kit;Mouse interleukin-7 ELISA Kit;

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