Mouse Il34 ELISA Kit

Alternative name
Mouse IL-34 ELISA Kit;Mouse 2010004A03Rik ELISA Kit;Mouse AI593503 ELISA Kit;Mouse interleukin 34 ELISA Kit;Mouse interleukin-34 ELISA Kit;
Catalog
E056370
species
Mouse
GeneIl34
SpecificityThe Mouse IL-34 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-34 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseMouse Il34 ELISA Kit allows for the in vitro quantitative determination of Il34 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Mouse IL-34 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-34 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-34 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-34 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-34 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-34 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.
Mouse Interleukin-34 Protein information
Uniprot ID IL34_MOUSE
Uniprot AC Q8R1R4; B2ZC71; B2ZC72; Q9D8F2;
UniGene Mm.77697;
GeneID 76527
KEGG mmu:76527;
Mouse Interleukin-34 Protein SEQUENCE
SEQUENCE 235 AA; 26764 MW; 4D30E33C977FD54D CRC64;
MPWGLAWLYC LGILLDVALG NENLEIWTLT QDKECDLTGY LRGKLQYKNR
LQYMKHYFPI NYRIAVPYEG VLRVANITRL QKAHVSEREL RYLWVLVSLN
ATESVMDVLL EGHPSWKYLQ EVQTLLENVQ RSLMDVEIGP HVEAVLSLLS
TPGLSLKLVR PKALLDNCFR VMELLYCSCC KQSPILKWQD CELPRLHPHS
PGSLMQCTAT NVYPLSRQTP TSLPGSPSSS HGSLP
UCSC uc012gko.1; mouse. [Q8R1R4-3];

Category

Mouse Il34 ELISA Kit

Mouse IL-34 ELISA Kit;Mouse 2010004A03Rik ELISA Kit;Mouse AI593503 ELISA Kit;Mouse interleukin 34 ELISA Kit;Mouse interleukin-34 ELISA Kit;