Mouse Il12a ELISA Kit

Alternative name
Mouse Il-12a ELISA Kit;Mouse cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit;Mouse CLMF p35 ELISA Kit;Mouse IL-12 subunit p35 ELISA Kit;Mouse IL-12p35 ELISA Kit;Mouse Ll12a ELISA Kit;Mouse p35 ELISA Kit;Mouse interleukin 12a ELISA Kit;Mouse interleukin-12 subunit alpha ELISA Kit;Mouse IL-12 p35 subunit ELISA Kit;Mouse interleukin 12a p35 subunit ELISA Kit;Mouse interleukin-12 p35 subunit ELISA Kit;
Catalog
E056283
species
Mouse
GeneIl12a
SpecificityThe Mouse IL-12 p70 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-12 p70 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7 pg/ml.
Intended UseMouse Il12a ELISA Kit allows for the in vitro quantitative determination of Il12a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Mouse IL-12 p70 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p70 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12 p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.
Mouse Interleukin-12 subunit alpha Protein information
Uniprot ID IL12A_MOUSE
Uniprot AC P43431;
UniGene Mm.103783;
GeneID 16159
KEGG mmu:16159;
Mouse Interleukin-12 subunit alpha Protein SEQUENCE
SEQUENCE 215 AA; 24179 MW; 56845B9B66D35A53 CRC64;
MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT
AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS
TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII
LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST
RVVTINRVMG YLSSA
UCSC uc008plv.3; mouse.;

Mouse Il12a ELISA Kit

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