Mouse Igfbp5 ELISA Kit

Alternative name
Mouse IBP-5 ELISA Kit;Mouse IGF-binding protein 5 ELISA Kit;Mouse IGFBP-5 ELISA Kit;Mouse AI256729 ELISA Kit;Mouse AW208790 ELISA Kit;Mouse IGFBP-5P ELISA Kit;Mouse insulin-like growth factor binding protein 5 ELISA Kit;Mouse insulin-like growth factor-binding protein 5 ELISA Kit;
Catalog
E056239
species
Mouse
GeneIgfbp5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse IGFBP5. No significant cross-reactivity or interference between Mouse IGFBP5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IGFBP5 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity75pg/mL
Intended UseMouse Igfbp5 ELISA Kit allows for the in vitro quantitative determination of Igfbp5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP5 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IGFBP5. You can calculate the concentration of Mouse IGFBP5 in the samples by comparing the OD of the samples to the standard curve.
Mouse Insulin-like growth factor-binding protein 5 Protein information
Uniprot ID IBP5_MOUSE
Uniprot AC Q07079;
UniGene Mm.405761;
GeneID 16011
KEGG mmu:16011;
Mouse Insulin-like growth factor-binding protein 5 Protein SEQUENCE
SEQUENCE 271 AA; 30372 MW; F55A58729861F6F0 CRC64;
MVISVVLLLL AAYAVPAQGL GSFVHCEPCD EKALSMCPPS PLGCELVKEP
GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL
NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA
VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESEQGP CRRHMEASLQ
EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL
PGMEYVDGDF QCHAFDSSNV E
UCSC uc007bkx.1; mouse.;

Category

Mouse Igfbp5 ELISA Kit

Mouse IBP-5 ELISA Kit;Mouse IGF-binding protein 5 ELISA Kit;Mouse IGFBP-5 ELISA Kit;Mouse AI256729 ELISA Kit;Mouse AW208790 ELISA Kit;Mouse IGFBP-5P ELISA Kit;Mouse insulin-like growth factor binding protein 5 ELISA Kit;Mouse insulin-like growth factor-binding protein 5 ELISA Kit;