Mouse Igfbp5 ELISA Kit

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  • Alternative name

    Mouse IBP-5 ELISA Kit;Mouse IGF-binding protein 5 ELISA Kit;Mouse IGFBP-5 ELISA Kit;Mouse AI256729 ELISA Kit;Mouse AW208790 ELISA Kit;Mouse IGFBP-5P ELISA Kit;Mouse insulin-like growth factor binding protein 5 ELISA Kit;Mouse insulin-like growth factor-binding protein 5 ELISA Kit;

  • Catalog
    E056239
  • species
    Mouse
  • GeneIgfbp5
  • Other Species Human IGFBP5 ELISA KitBovine IGFBP5 ELISA KitPorcine IGFBP5 ELISA KitRat Igfbp5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse IGFBP5. No significant cross-reactivity or interference between Mouse IGFBP5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IGFBP5 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity75pg/mL
  • Intended UseMouse Igfbp5 ELISA Kit allows for the in vitro quantitative determination of Igfbp5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP5 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IGFBP5. You can calculate the concentration of Mouse IGFBP5 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Insulin-like growth factor-binding protein 5 Protein information
  • Uniprot ID IBP5_MOUSE
  • Uniprot AC Q07079;
  • UniGene Mm.405761;
  • GeneID 16011
  • KEGG mmu:16011;
  • Mouse Insulin-like growth factor-binding protein 5 Protein SEQUENCE
  • SEQUENCE 271 AA; 30372 MW; F55A58729861F6F0 CRC64;

    MVISVVLLLL AAYAVPAQGL GSFVHCEPCD EKALSMCPPS PLGCELVKEP

    GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL

    NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA

    VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESEQGP CRRHMEASLQ

    EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL

    PGMEYVDGDF QCHAFDSSNV E

  • UCSC uc007bkx.1; mouse.;



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