Mouse Igfbp1 ELISA Kit

Alternative name
Mouse IBP-1 ELISA Kit;Mouse IGF-binding protein 1 ELISA Kit;Mouse Igfbp-1 ELISA Kit;Mouse insulin-like growth factor binding protein 1 ELISA Kit;Mouse insulin-like growth factor-binding protein 1 ELISA Kit;Mouse INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1) ELISA Kit;
Catalog
E056235
species
Mouse
GeneIgfbp1
SpecificityThe Mouse IGFBP1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IGFBP1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Mouse IGFBP1 is typically less than 3 pg/ml.
Intended UseMouse Igfbp1 ELISA Kit allows for the in vitro quantitative determination of Igfbp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The Mouse IGFBP1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IGFBP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7.
Mouse Insulin-like growth factor-binding protein 1 Protein information
Uniprot ID IBP1_MOUSE
Uniprot AC P47876; Q5SVY8; Q61732;
UniGene Mm.21300;
GeneID 16006
KEGG mmu:16006;
Mouse Insulin-like growth factor-binding protein 1 Protein SEQUENCE
SEQUENCE 272 AA; 29570 MW; CD4FCD3C5AB69B60 CRC64;
MPEFLTVVSW PFLILLSFQI GVAAGAPQPW HCAPCTAERL GLCPPVPASC
PEISRPAGCG CCPTCALPMG AACGVATARC AQGLSCRALP GEPRPLHALT
RGQGACVPEP AAPATSTLFS SQHEEAKAAV VSADELSESP EMTEEQLLDS
FHLMAPSRED QPILWNAIST YSSMRAREIA DLKKWKEPCQ RELYKVLERL
AAAQQKAGDE IYKFYLPNCN KNGFYHSKQC ETSLDGEAGL CWCVYPWSGK
KIPGSLETRG DPNCHQYFNV HN
UCSC uc007hzh.2; mouse.;

Mouse Igfbp1 ELISA Kit

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