Mouse Igf1 ELISA Kit

Alternative name
Mouse Igf-I ELISA Kit;Mouse somatomedin ELISA Kit;Mouse Igf-1 ELISA Kit;Mouse C730016P09Rik ELISA Kit;Mouse insulin-like growth factor 1 ELISA Kit;Mouse insulin-like growth factor I ELISA Kit;
Catalog
E056225
species
Mouse
GeneIgf1
SpecificityNatural and recombinant mouse IGF-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 5pg/ml
Intended UseMouse Igf1 ELISA Kit allows for the in vitro quantitative determination of Igf1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGF-1has been precoated onto 96-well plates. Standards(E.coli,G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGF-1 amount of sample captured in plate. Background: Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A).2The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation.3 IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in aparacrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Mouse Insulin-like growth factor I Protein information
Uniprot ID IGF1_MOUSE
Uniprot AC P05017; P05018; Q6LDP4; Q8C4U6;
UniGene Mm.268521;
GeneID 16000
KEGG mmu:16000;
Mouse Insulin-like growth factor I Protein SEQUENCE
SEQUENCE 153 AA; 17093 MW; 967596AEAC0CA387 CRC64;
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP
ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC
DLRRLEMYCA PLKPTKAARS IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT
YRM
UCSC uc007gqw.2; mouse. [P05017-1];

Mouse Igf1 ELISA Kit

Mouse Igf-I ELISA Kit;Mouse somatomedin ELISA Kit;Mouse Igf-1 ELISA Kit;Mouse C730016P09Rik ELISA Kit;Mouse insulin-like growth factor 1 ELISA Kit;Mouse insulin-like growth factor I ELISA Kit;

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