Mouse Hig2 ELISA Kit

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  • Alternative name

    Hypoxia-inducible lipid droplet-associated protein ELISA KIT; Hypoxia-inducible gene 2 proteinHilpda ELISA KIT; Hig2 ELISA KIT

  • Catalog
  • species
  • GeneHig2
  • Standard CurveMouse Hig2 ELISA Kit
  • Other Species Human HIG2 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse Hypoxia-inducible gene 2 protein. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Hig2 ELISA Kit allows for the in vitro quantitative determination of Hig2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia-inducible gene 2 protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hypoxia-inducible gene 2 protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hypoxia-inducible gene 2 protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Hypoxia-inducible gene 2 protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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