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Alternative name
RAF proto-oncogene serine/threonine-protein kinase ELISA KIT; Proto-oncogene c-RAF ELISA KIT; cRaf ELISA KIT; Raf-1Raf1 ELISA KIT; Craf ELISA KIT; cRaf ELISA KIT
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Catalog
E005597
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species
Human
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GeneCRAF
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Standard Curve
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Other Species
Mouse CRAF ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF).
No significant cross-reactivity or interference between C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.059ng/mL.
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Intended UseHuman CRAF ELISA Kit allows for the in vitro quantitative determination of CRAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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