Mouse Hint2 ELISA Kit

Alternative name
Mouse HINT-2 ELISA Kit;Mouse HINT-3 ELISA Kit;Mouse 1190005L05Rik ELISA Kit;Mouse histidine triad nucleotide binding protein 2 ELISA Kit;Mouse histidine triad nucleotide-binding protein 2, mitochondrial ELISA Kit;
Catalog
E055813
species
Mouse
GeneHint2
Standard Curve
Other Species Human HINT2 ELISA Kit Bovine HINT2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Triad Nucleotide Binding Protein 2 (HINT2). No significant cross-reactivity or interference between Histidine Triad Nucleotide Binding Protein 2 (HINT2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseMouse Hint2 ELISA Kit allows for the in vitro quantitative determination of Hint2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Triad Nucleotide Binding Protein 2 (HINT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Triad Nucleotide Binding Protein 2 (HINT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Triad Nucleotide Binding Protein 2 (HINT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Triad Nucleotide Binding Protein 2 (HINT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Histidine triad nucleotide-binding protein 2, mitochondrial Protein information
Uniprot ID HINT2_MOUSE
Uniprot AC Q9D0S9;
UniGene Mm.25285;
GeneID 68917
KEGG mmu:68917;
Mouse Histidine triad nucleotide-binding protein 2, mitochondrial Protein SEQUENCE
SEQUENCE 163 AA; 17320 MW; 1FD421AF2AD3E448 CRC64;
MAAAVLLAVG LRAARRTLAA AGARGAQVRG NAGVSDGSEV AKAQKAAPGG
ASPTIFSRIL DRSLPADILY EDQQCLVFRD VAPQAPVHFL VIPRKPIPRI
SQAEEDDQQL LGHLLLVAKK IAQAQGLKDG YRLVVNDGKM GAQSVYHLHI
HVLGGRQLQW PPG
UCSC uc008sqs.1; mouse.;

Category

Mouse Hint2 ELISA Kit

Mouse HINT-2 ELISA Kit;Mouse HINT-3 ELISA Kit;Mouse 1190005L05Rik ELISA Kit;Mouse histidine triad nucleotide binding protein 2 ELISA Kit;Mouse histidine triad nucleotide-binding protein 2, mitochondrial ELISA Kit;