Mouse Hk2 ELISA Kit

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  • Alternative name

    Mouse hexokinase type II ELISA Kit;Mouse HK II ELISA Kit;Mouse AI642394 ELISA Kit;Mouse HKII ELISA Kit;Mouse hexokinase 2 ELISA Kit;Mouse hexokinase-2 ELISA Kit;

  • Catalog
    E055695
  • species
    Mouse
  • GeneHk2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HK2. No significant cross-reactivity or interference between HK2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.063ng/mL.
  • Intended UseMouse Hk2 ELISA Kit allows for the in vitro quantitative determination of Hk2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HK2 in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to HK2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Hexokinase-2 Protein information
  • Uniprot ID HXK2_MOUSE
  • Uniprot AC O08528;
  • UniGene Mm.255848; Mm.469167;
  • GeneID 15277
  • KEGG mmu:15277;
  • Mouse Hexokinase-2 Protein SEQUENCE
  • SEQUENCE 917 AA; 102535 MW; 44293E7A92D724B3 CRC64;

    MIASHMIACL FTELNQNQVQ KVDQYLYHMR LSDETLLEIS RRFRKEMEKG

    LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN

    GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL

    GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD

    IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG

    DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM

    YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEDDKDGIQ

    KAYQILVRLG LSPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN

    KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG

    KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS

    KETHEAAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR

    RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF

    TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IRRREEFDLD

    VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE

    GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL

    GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV

    RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG

    LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG

    AALITAVACR IREAGQR

  • UCSC uc009cll.2; mouse.;



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