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Alternative name
Hepatocyte nuclear factor 4-alpha ELISA KIT; Nuclear receptor subfamily 2 group A member 1 ELISA KIT; Transcription factor 14 ELISA KIT; TCF-14 ELISA KIT; Transcription factor HNF-4HNF4A ELISA KIT; HNF4 ELISA KIT; NR2A1 ELISA KIT; TCF14 ELISA KIT; HNF-4-alpha ELISA KIT; TCF-14 ELISA KIT
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Catalog
E055595
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species
Mouse
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GeneHNF4alpha
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Standard Curve
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Other Species
Human HNF4Alpha ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse HNF4alpha ELISA Kit allows for the in vitro quantitative determination of HNF4alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyEpigenetics and Nuclear Signaling
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Product Description
specificalFor Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This HNF4alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HNF4alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: HNF4alpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HNF4alpha. Standards or samples are then added to the microtiter plate wells and HNF4alpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HNF4alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HNF4alpha are added to each well to "sandwich" the HNF4alpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HNF4alpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HNF4alpha concentration in each sample is interpolated from this standard curve.
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