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Mouse Hmox1 ELISA Kit

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  • Alternative name

    Mouse HO-1 ELISA Kit;Mouse P32 protein ELISA Kit;Mouse D8Wsu38e ELISA Kit;Mouse HO1 ELISA Kit;Mouse Hemox ELISA Kit;Mouse Hmox ELISA Kit;Mouse Hsp32 ELISA Kit;Mouse heme oxygenase 1 ELISA Kit;Mouse heme oxygenase (decycling) 1 ELISA Kit;Mouse hemoxygenase ELISA Kit;
  • Catalog
    E055410
  • species
    Mouse
  • GeneHmox1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse HMOX1. No significant cross-reactivity or interference between Mouse HMOX1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity7.8 pg/ml
  • Intended UseMouse Hmox1 ELISA Kit allows for the in vitro quantitative determination of Hmox1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant. HO1 expression is greatly increased in neurofibrillary tangles in Alzheimer's disease and other neurodegenerative diseases such as progressive supranuclear palsy and subacute sclerosing panencephalitis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HO-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HO-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Heme oxygenase 1 Protein information
  • Uniprot ID HMOX1_MOUSE
  • Uniprot AC P14901;
  • UniGene Mm.276389;
  • GeneID 15368
  • KEGG mmu:15368;
  • Mouse Heme oxygenase 1 Protein SEQUENCE

  • SEQUENCE 289 AA; 32929 MW; 19DB0DCCD574044B CRC64;

    MERPQPDSMP QDLSEALKEA TKEVHIQAEN AEFMKNFQKG QVSREGFKLV

    MASLYHIYTA LEEEIERNKQ NPVYAPLYFP EELHRRAALE QDMAFWYGPH

    WQEIIPCTPA TQHYVKRLHE VGRTHPELLV AHAYTRYLGD LSGGQVLKKI

    AQKAMALPSS GEGLAFFTFP NIDSPTKFKQ LYRARMNTLE MTPEVKHRVT

    EEAKTAFLLN IELFEELQVM LTEEHKDQSP SQMASLRQRP ASLVQDTAPA

    ETPRGKPQIS TSSSQTPLLQ WVLTLSFLLA TVAVGIYAM

  • UCSC uc009mhc.2; mouse.;



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