Mouse Hmox1 ELISA Kit

Alternative name
Mouse HO-1 ELISA Kit;Mouse P32 protein ELISA Kit;Mouse D8Wsu38e ELISA Kit;Mouse HO1 ELISA Kit;Mouse Hemox ELISA Kit;Mouse Hmox ELISA Kit;Mouse Hsp32 ELISA Kit;Mouse heme oxygenase 1 ELISA Kit;Mouse heme oxygenase (decycling) 1 ELISA Kit;Mouse hemoxygenase ELISA Kit;
Catalog
E055410
species
Mouse
GeneHmox1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse HMOX1. No significant cross-reactivity or interference between Mouse HMOX1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7.8 pg/ml
Intended UseMouse Hmox1 ELISA Kit allows for the in vitro quantitative determination of Hmox1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant. HO1 expression is greatly increased in neurofibrillary tangles in Alzheimer's disease and other neurodegenerative diseases such as progressive supranuclear palsy and subacute sclerosing panencephalitis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HO-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HO-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Heme oxygenase 1 Protein information
Uniprot ID HMOX1_MOUSE
Uniprot AC P14901;
UniGene Mm.276389;
GeneID 15368
KEGG mmu:15368;
Mouse Heme oxygenase 1 Protein SEQUENCE
SEQUENCE 289 AA; 32929 MW; 19DB0DCCD574044B CRC64;
MERPQPDSMP QDLSEALKEA TKEVHIQAEN AEFMKNFQKG QVSREGFKLV
MASLYHIYTA LEEEIERNKQ NPVYAPLYFP EELHRRAALE QDMAFWYGPH
WQEIIPCTPA TQHYVKRLHE VGRTHPELLV AHAYTRYLGD LSGGQVLKKI
AQKAMALPSS GEGLAFFTFP NIDSPTKFKQ LYRARMNTLE MTPEVKHRVT
EEAKTAFLLN IELFEELQVM LTEEHKDQSP SQMASLRQRP ASLVQDTAPA
ETPRGKPQIS TSSSQTPLLQ WVLTLSFLLA TVAVGIYAM
UCSC uc009mhc.2; mouse.;

Mouse Hmox1 ELISA Kit

Mouse HO-1 ELISA Kit;Mouse P32 protein ELISA Kit;Mouse D8Wsu38e ELISA Kit;Mouse HO1 ELISA Kit;Mouse Hemox ELISA Kit;Mouse Hmox ELISA Kit;Mouse Hsp32 ELISA Kit;Mouse heme oxygenase 1 ELISA Kit;Mouse heme oxygenase (decycling) 1 ELISA Kit;Mouse hemoxygenase ELISA Kit;

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