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Mouse Hspa1a ELISA Kit

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  • Alternative name

    Mouse heat shock 70 kDa protein 3 ELISA Kit;Mouse Hsp70.3 ELISA Kit;Mouse hsp68 ELISA Kit;Mouse Hsp70-3 ELISA Kit;Mouse hsp70A1 ELISA Kit;Mouse Hsp72 ELISA Kit;Mouse heat shock protein 1A ELISA Kit;Mouse heat shock 70 kDa protein 1A ELISA Kit;Mouse 68 kDa heat shock protein ELISA Kit;Mouse heat shock 70kDa protein 1A ELISA Kit;Mouse heat shock protein, 70 kDa 3 ELISA Kit;Mouse inducible heat shock protein 70 ELISA Kit;
  • Catalog
    E055245
  • species
    Mouse
  • GeneHspa1a
  • Other Species Human HSPA1A ELISA KitBovine HSPA1A ELISA KitPorcine HSPA1A ELISA KitRat Hspa1a ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseMouse Hspa1a ELISA Kit allows for the in vitro quantitative determination of Hspa1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Hspa1a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Hspa1a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Hspa1a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Hspa1a can be calculated.
  • Mouse Heat shock 70 kDa protein 1A Protein information
  • Uniprot ID HS71A_MOUSE
  • Uniprot AC Q61696; Q61697; Q7TQD8; Q9QWJ5;
  • UniGene Mm.6388;
  • GeneID 193740
  • KEGG mmu:193740;
  • Mouse Heat shock 70 kDa protein 1A Protein SEQUENCE

  • SEQUENCE 641 AA; 70079 MW; F49C33E602EAE334 CRC64;

    MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL

    IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD AVVQSDMKHW PFQVVNDGDK

    PKVQVNYKGE SRSFFPEEIS SMVLTKMKEI AEAYLGHPVT NAVITVPAYF

    NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL

    GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVSH FVEEFKRKHK

    KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA

    RFEELCSDLF RGTLEPVEKA LRDAKMDKAQ IHDLVLVGGS TRIPKVQKLL

    QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS

    LGLETAGGVM TALIKRNSTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT

    RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK

    ITITNDKGRL SKEEIERMVQ EAERYKAEDE VQRDRVAAKN ALESYAFNMK

    SAVEDEGLKG KLSEADKKKV LDKCQEVISW LDSNTLADKE EFVHKREELE

    RVCSPIISGL YQGAGAPGAG GFGAQAPKGA SGSGPTIEEV D

  • UCSC uc008cep.1; mouse.;


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