Mouse Spint2 ELISA Kit

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  • Alternative name

    Mouse hepatocyte growth factor activator inhibitor type 2 ELISA Kit;Mouse HAI-2 ELISA Kit;Mouse Hai2 ELISA Kit;Mouse AL024025 ELISA Kit;Mouse C76321 ELISA Kit;Mouse serine protease inhibitor, Kunitz type 2 ELISA Kit;Mouse kunitz-type protease inhibitor 2 ELISA Kit;

  • Catalog
    E055172
  • species
    Mouse
  • GeneSpint2
  • Standard CurveMouse Spint2 ELISA Kit
  • Other Species Human SPINT2 ELISA Kit
  • SpecificityNatural and recombinant Mouse HAI-2/SPINT2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
  • Intended UseMouse Spint2 ELISA Kit allows for the in vitro quantitative determination of Spint2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Mouse HAI-2/SPINT2 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for HAI-2/SPINT2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: R30-K140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HAI-2/SPINT2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HAI-2/SPINT2 amount of sample captured in plate. Background/Introduction: Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Mouse Kunitz-type protease inhibitor 2 Protein information
  • Uniprot ID SPIT2_MOUSE
  • Uniprot AC Q9WU03; Q5D0F2; Q9WU04; Q9WU05;
  • UniGene Mm.295230;
  • GeneID 20733
  • KEGG mmu:20733;
  • Mouse Kunitz-type protease inhibitor 2 Protein SEQUENCE
  • SEQUENCE 252 AA; 27914 MW; B2FF4B86924D4F8F CRC64;

    MAQLCELRRG RALLALVASL LLSGAQVASR ELDVHESCGV SKVVGKCRAS

    IPRWWYNITD GSCQPFVYGG CEGNGNNYQS KEECLDKCAG VTENTTDDMA

    RNRNGADSSV LSVPRKQSAE DLSAEIFNYE EYCVPKAVTG PCRAAFPRWY

    YDTEKNSCIS FIYGGCRGNK NSYLSQEACM QHCSGKQMHP FLTPGLKAVI

    LVGLFLMVLI LLLGTSMVCL IRVVRRKQER ALRTVWSTAD DKEQLVKNTC

    VL

  • UCSC uc009gbl.1; mouse. [Q9WU03-2];



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