Human CLEC7A ELISA Kit

Alternative name
Human beta-glucan receptor ELISA Kit;Human C-type lectin superfamily member 12 ELISA Kit;Human Dendritic cell-associated C-type lectin 1 ELISA Kit;Human DC-associated C-type lectin 1 ELISA Kit;Human dectin-1 ELISA Kit;Human BGR ELISA Kit;Human CLECSF12 ELISA Kit;Human DECTIN1 ELISA Kit;Human CANDF4 ELISA Kit;Human CD369 ELISA Kit;Human SCARE2 ELISA Kit;Human C-type lectin domain family 7 member A ELISA Kit;Human C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12 ELISA Kit;Human dendritic cell-associated C-type lectin-1 ELISA Kit;Human lectin-like receptor 1 ELISA Kit;
Catalog
E005499
species
Human
GeneCLEC7A
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLEC7A. No significant cross-reactivity or interference between CLEC7A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC7A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLEC7A ELISA Kit allows for the in vitro quantitative determination of CLEC7A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CLEC7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC7A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CLEC7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLEC7A antibody and an CLEC7A-HRP conjugate. The assay sample and buffer are incubated together with CLEC7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC7A concentration since CLEC7A from samples and CLEC7A-HRP conjugate compete for the anti-CLEC7A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC7A from the sample, fewer sites are left to bind CLEC7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC7A concentration in each sample is interpolated from this standard curve.
Human C-type lectin domain family 7 member A Protein information
Uniprot ID CLC7A_HUMAN
Uniprot AC Q96LD3; Q96PA4; Q96PA5; Q96PA6; Q96PA7; Q96PA8; Q9H1K3;
UniGene Hs.143929;
GeneID 64581
KEGG hsa:64581;
Human C-type lectin domain family 7 member A Protein SEQUENCE
SEQUENCE 247 AA; 27627 MW; 98393E36976111B9 CRC64;
MEYHPDLENL DEDGYTQLHF DSQSNTRIAV VSEKGSCAAS PPWRLIAVIL
GILCLVILVI AVVLGTMAIW RSNSGSNTLE NGYFLSRNKE NHSQPTQSSL
EDSVTPTKAV KTTGVLSSPC PPNWIIYEKS CYLFSMSLNS WDGSKRQCWQ
LGSNLLKIDS SNELGFIVKQ VSSQPDNSFW IGLSRPQTEV PWLWEDGSTF
SSNLFQIRTT ATQENPSPNC VWIHVSVIYD QLCSVPSYSI CEKKFSM
UCSC uc001qxe.5; human. [Q9BXN2-1];

Human CLEC7A ELISA Kit

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