Human CLEC7A ELISA Kit

Alternative name
Human beta-glucan receptor ELISA Kit;Human C-type lectin superfamily member 12 ELISA Kit;Human Dendritic cell-associated C-type lectin 1 ELISA Kit;Human DC-associated C-type lectin 1 ELISA Kit;Human dectin-1 ELISA Kit;Human BGR ELISA Kit;Human CLECSF12 ELISA Kit;Human DECTIN1 ELISA Kit;Human CANDF4 ELISA Kit;Human CD369 ELISA Kit;Human SCARE2 ELISA Kit;Human C-type lectin domain family 7 member A ELISA Kit;Human C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12 ELISA Kit;Human dendritic cell-associated C-type lectin-1 ELISA Kit;Human lectin-like receptor 1 ELISA Kit;
Catalog
E005499
species
Human
GeneCLEC7A
Standard Curve
Other Species Mouse Clec7a ELISA Kit Bovine CLEC7A ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLEC7A. No significant cross-reactivity or interference between CLEC7A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC7A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLEC7A ELISA Kit allows for the in vitro quantitative determination of CLEC7A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CLEC7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC7A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CLEC7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLEC7A antibody and an CLEC7A-HRP conjugate. The assay sample and buffer are incubated together with CLEC7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC7A concentration since CLEC7A from samples and CLEC7A-HRP conjugate compete for the anti-CLEC7A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC7A from the sample, fewer sites are left to bind CLEC7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC7A concentration in each sample is interpolated from this standard curve.
Human C-type lectin domain family 7 member A Protein information
Uniprot ID CLC7A_HUMAN
Uniprot AC Q96LD3; Q96PA4; Q96PA5; Q96PA6; Q96PA7; Q96PA8; Q9H1K3;
UniGene Hs.143929;
GeneID 64581
KEGG hsa:64581;
Human C-type lectin domain family 7 member A Protein SEQUENCE
SEQUENCE 247 AA; 27627 MW; 98393E36976111B9 CRC64;
MEYHPDLENL DEDGYTQLHF DSQSNTRIAV VSEKGSCAAS PPWRLIAVIL
GILCLVILVI AVVLGTMAIW RSNSGSNTLE NGYFLSRNKE NHSQPTQSSL
EDSVTPTKAV KTTGVLSSPC PPNWIIYEKS CYLFSMSLNS WDGSKRQCWQ
LGSNLLKIDS SNELGFIVKQ VSSQPDNSFW IGLSRPQTEV PWLWEDGSTF
SSNLFQIRTT ATQENPSPNC VWIHVSVIYD QLCSVPSYSI CEKKFSM
UCSC uc001qxe.5; human. [Q9BXN2-1];

Category

Human CLEC7A ELISA Kit