Human CLEC2D ELISA Kit

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  • Alternative name

    Human lectin-like NK cell receptor ELISA Kit;Human lectin-like transcript 1 ELISA Kit;Human LLT-1 ELISA Kit;Human osteoclast inhibitory lectin ELISA Kit;Human CLAX ELISA Kit;Human LLT1 ELISA Kit;Human OCIL ELISA Kit;Human C-type lectin domain family 2 member D ELISA Kit;Human C-type lectin related f ELISA Kit;Human C-type lectin superfamily 2, member D ELISA Kit;

  • Catalog
    E005486
  • species
    Human
  • GeneCLEC2D
  • Standard CurveHuman CLEC2D ELISA Kit
  • Other Species Mouse Clec2d ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLEC2D. No significant cross-reactivity or interference between CLEC2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC2D and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CLEC2D ELISA Kit allows for the in vitro quantitative determination of CLEC2D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: CLEC2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC2D antibody and an CLEC2D-HRP conjugate. The assay sample and buffer are incubated together with CLEC2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC2D concentration since CLEC2D from samples and CLEC2D-HRP conjugate compete for the anti-CLEC2D antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC2D from the sample, fewer sites are left to bind CLEC2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC2D concentration in each sample is interpolated from this standard curve.
  • Human C-type lectin domain family 2 member D Protein information
  • Uniprot ID CLC2D_HUMAN
  • Uniprot AC Q9UHP7; D6CI39; D6CI40; D6CI41; Q6YID5; Q8WUP7; Q9HD37; Q9HD38;
  • UniGene Hs.268326;
  • GeneID 29121
  • KEGG hsa:29121;
  • Human C-type lectin domain family 2 member D Protein SEQUENCE
  • SEQUENCE 191 AA; 21849 MW; E71F3E21DC361F10 CRC64;

    MHDSNNVEKD ITPSELPANP GCLHSKEHSI KATLIWRLFF LIMFLTIIVC

    GMVAALSAIR ANCHQEPSVC LQAACPESWI GFQRKCFYFS DDTKNWTSSQ

    RFCDSQDADL AQVESFQELN FLLRYKGPSD HWIGLSREQG QPWKWINGTE

    WTRQFPILGA GECAYLNDKG ASSARHYTER KWICSKSDIH V

  • UCSC uc001qwf.4; human. [Q9UHP7-1];


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