Human CLEC2D ELISA Kit

Alternative name
Human lectin-like NK cell receptor ELISA Kit;Human lectin-like transcript 1 ELISA Kit;Human LLT-1 ELISA Kit;Human osteoclast inhibitory lectin ELISA Kit;Human CLAX ELISA Kit;Human LLT1 ELISA Kit;Human OCIL ELISA Kit;Human C-type lectin domain family 2 member D ELISA Kit;Human C-type lectin related f ELISA Kit;Human C-type lectin superfamily 2, member D ELISA Kit;
Catalog
E005486
species
Human
GeneCLEC2D
Standard Curve
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLEC2D. No significant cross-reactivity or interference between CLEC2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC2D and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLEC2D ELISA Kit allows for the in vitro quantitative determination of CLEC2D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CLEC2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC2D antibody and an CLEC2D-HRP conjugate. The assay sample and buffer are incubated together with CLEC2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC2D concentration since CLEC2D from samples and CLEC2D-HRP conjugate compete for the anti-CLEC2D antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC2D from the sample, fewer sites are left to bind CLEC2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC2D concentration in each sample is interpolated from this standard curve.
Human C-type lectin domain family 2 member D Protein information
Uniprot ID CLC2D_HUMAN
Uniprot AC Q9UHP7; D6CI39; D6CI40; D6CI41; Q6YID5; Q8WUP7; Q9HD37; Q9HD38;
UniGene Hs.268326;
GeneID 29121
KEGG hsa:29121;
Human C-type lectin domain family 2 member D Protein SEQUENCE
SEQUENCE 191 AA; 21849 MW; E71F3E21DC361F10 CRC64;
MHDSNNVEKD ITPSELPANP GCLHSKEHSI KATLIWRLFF LIMFLTIIVC
GMVAALSAIR ANCHQEPSVC LQAACPESWI GFQRKCFYFS DDTKNWTSSQ
RFCDSQDADL AQVESFQELN FLLRYKGPSD HWIGLSREQG QPWKWINGTE
WTRQFPILGA GECAYLNDKG ASSARHYTER KWICSKSDIH V
UCSC uc001qwf.4; human. [Q9UHP7-1];

Human CLEC2D ELISA Kit

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