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Mouse Gzmb ELISA Kit

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  • Alternative name

    Mouse Ctla-1 ELISA Kit;Mouse cytotoxic cell protease 1 ELISA Kit;Mouse CCP1 ELISA Kit;Mouse fragmentin-2 ELISA Kit;Mouse Ctla1 ELISA Kit;Mouse AI553453 ELISA Kit;Mouse CCP-1/C11 ELISA Kit;Mouse GZB ELISA Kit;Mouse granzyme B ELISA Kit;Mouse granzyme B(G,H) ELISA Kit;
  • Catalog
    E054809
  • species
    Mouse
  • GeneGzmb
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GZMB. No significant cross-reactivity or interference between Mouse GZMB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.08 ng/ml
  • Intended UseMouse Gzmb ELISA Kit allows for the in vitro quantitative determination of Gzmb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gzms-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Gzms-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Gzms-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gzms-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Granzyme B(G,H) Protein information
  • Uniprot ID GRAB_MOUSE
  • Uniprot AC P04187;
  • UniGene Mm.14874;
  • GeneID 14939
  • KEGG mmu:14939;
  • Mouse Granzyme B(G,H) Protein SEQUENCE

  • SEQUENCE 247 AA; 27470 MW; 996BCD199965C6D6 CRC64;

    MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG

    GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY

    NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM

    APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR

    GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS

  • UCSC uc007ubv.1; mouse.;



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