Mouse Gpc4 ELISA Kit

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  • Alternative name

    Mouse K-glypican ELISA Kit;Mouse 9530073D23Rik ELISA Kit;Mouse AF311610 ELISA Kit;Mouse AI385680 ELISA Kit;Mouse AI661372 ELISA Kit;Mouse glypican 4 ELISA Kit;Mouse glypican-4 ELISA Kit;

  • Catalog
    E054698
  • species
    Mouse
  • GeneGpc4
  • Standard CurveMouse Gpc4 ELISA Kit
  • Other Species Human GPC4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GPC4. No significant cross-reactivity or interference between GPC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPC4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Gpc4 ELISA Kit allows for the in vitro quantitative determination of Gpc4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: GPC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC4 antibody and an GPC4-HRP conjugate. The assay sample and buffer are incubated together with GPC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC4 concentration since GPC4 from samples and GPC4-HRP conjugate compete for the anti-GPC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC4 from the sample, fewer sites are left to bind GPC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC4 concentration in each sample is interpolated from this standard curve.
  • Mouse Secreted glypican-4 Protein information
  • Uniprot ID GPC4_MOUSE
  • Uniprot AC P51655; Q923C8;
  • UniGene Mm.1528; Mm.478164; Mm.491492;
  • GeneID 14735
  • KEGG mmu:14735;
  • Mouse Secreted glypican-4 Protein SEQUENCE
  • SEQUENCE 557 AA; 62586 MW; F216AA0FEF5ED840 CRC64;

    MARLGLLALL CTLAALSASL LAAELKSKSC SEVRRLYVSK GFNKNDAPLY

    EINGDHLKIC PQDYTCCSQE MEEKYSLQSK DDFKTVVSEQ CNHLQAIFAS

    RYKKFDEFFK ELLENAEKSL NDMFVKTYGH LYMQNSELFK DLFVELKRYY

    VAGNVNLEEM LNDFWARLLE RMFRLVNSQY HFTDEYLECV SKYTEQLKPF

    GDVPRKLKLQ VTRAFVAART FAQGLAVARD VVSKVSVVNP TAQCTHALLK

    MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE

    RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG

    RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW

    SSLPSTVCND ERMAAGNENE DDCWNGKGKS RYLFAVTGNG LANQGNNPEV

    QVDTSKPDIL ILRQIMALRV MTSKMKNAYN GNDVDFFDIS DESSGEGSGS

    GCEYQQCPSE FEYNATDHSG KSANEKADSA GGAHAEAKPY LLAALCILFL

    AVQGEWR

  • UCSC uc012hhb.1; mouse.;


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