Mouse Gstt2 ELISA Kit

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  • Alternative name

    Mouse GST class-theta-2 ELISA Kit;Mouse AI266894 ELISA Kit;Mouse Yrs ELISA Kit;Mouse mGSTT2 ELISA Kit;Mouse glutathione S-transferase, theta 2 ELISA Kit;Mouse glutathione S-transferase theta-2 ELISA Kit;

  • Catalog
    E054511
  • species
    Mouse
  • GeneGstt2
  • Other Species Human GSTT2 ELISA KitHuman GSTtheta2/GSTt2 ELISA KitRat Gstt2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Gstt2 ELISA Kit allows for the in vitro quantitative determination of Gstt2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: GSTT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSTT2 antibody and an GSTT2-HRP conjugate. The assay sample and buffer are incubated together with GSTT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSTT2 concentration since GSTT2 from samples and GSTT2-HRP conjugate compete for the anti-GSTT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GSTT2 from the sample, fewer sites are left to bind GSTT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSTT2 concentration in each sample is interpolated from this standard curve.
  • Mouse Glutathione S-transferase theta-2 Protein information
  • Uniprot ID GSTT2_MOUSE
  • Uniprot AC Q61133; Q61134; Q64472; Q91VB0;
  • UniGene Mm.24118;
  • GeneID 14872
  • KEGG mmu:14872;
  • Mouse Glutathione S-transferase theta-2 Protein SEQUENCE
  • SEQUENCE 244 AA; 27634 MW; 3F45D6E12DA3DF29 CRC64;

    MGLELYLDLL SQPSRAVYIF AKKNGIPFQT RTVDILKGQH MSEQFSQVNC

    LNKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG

    WHADNIRGTF GVLLWTKVLG PLIGVQVPQE KVERNRDRMV LVLQQLEDKF

    LRDRAFLVGQ QVTLADLMSL EELMQPVALG YNLFEGRPQL TAWRERVEAF

    LGAELCQEAH STILSILGQA AKKMLPVPPP EVHASMQLRI ARIP

  • UCSC uc011xgs.1; mouse.;



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