Human BCHE ELISA Kit

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  • Alternative name

    Human acylcholine acylhydrolase ELISA Kit;Human butyrylcholine esterase ELISA Kit;Human choline esterase II ELISA Kit;Human pseudocholinesterase ELISA Kit;Human CHE1 ELISA Kit;Human CHE2 ELISA Kit;Human E1 ELISA Kit;Human butyrylcholinesterase ELISA Kit;Human cholinesterase ELISA Kit;Human cholinesterase (serum) 2 ELISA Kit;Human cholinesterase 1 ELISA Kit;

  • Catalog
    E005439
  • species
    Human
  • GeneBCHE
  • Standard CurveHuman BCHE ELISA Kit
  • Other Species Mouse Bche ELISA KitBovine BCHE ELISA KitCanine BCHE ELISA KitHorse BCHE ELISA KitPorcine BCHE ELISA KitRabbit BCHE ELISA KitSheep BCHE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Butyrylcholinesterase (BCHE). No significant cross-reactivity or interference between Butyrylcholinesterase (BCHE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.115ng/mL.
  • Intended UseHuman BCHE ELISA Kit allows for the in vitro quantitative determination of BCHE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Butyrylcholinesterase (BCHE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Butyrylcholinesterase (BCHE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Butyrylcholinesterase (BCHE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Butyrylcholinesterase (BCHE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cholinesterase Protein information
  • Uniprot ID CHLE_HUMAN
  • Uniprot AC P06276; A8K7P8;
  • UniGene Hs.420483;
  • GeneID 590
  • KEGG hsa:590;
  • Human Cholinesterase Protein SEQUENCE
  • SEQUENCE 602 AA; 68418 MW; C9836409D9057F27 CRC64;

    MHSKVTIICI RFLFWFLLLC MLIGKSHTED DIIIATKNGK VRGMNLTVFG

    GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW SDIWNATKYA NSCCQNIDQS

    FPGFHGSEMW NPNTDLSEDC LYLNVWIPAP KPKNATVLIW IYGGGFQTGT

    SSLHVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEA PGNMGLFDQQ

    LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPG SHSLFTRAIL

    QSGSFNAPWA VTSLYEARNR TLNLAKLTGC SRENETEIIK CLRNKDPQEI

    LLNEAFVVPY GTPLSVNFGP TVDGDFLTDM PDILLELGQF KKTQILVGVN

    KDEGTAFLVY GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH

    YTDWVDDQRP ENYREALGDV VGDYNFICPA LEFTKKFSEW GNNAFFYYFE

    HRSSKLPWPE WMGVMHGYEI EFVFGLPLER RDNYTKAEEI LSRSIVKRWA

    NFAKYGNPNE TQNNSTSWPV FKSTEQKYLT LNTESTRIMT KLRAQQCRFW

    TSFFPKVLEM TGNIDEAEWE WKAGFHRWNN YMMDWKNQFN DYTSKKESCV

    GL

  • UCSC uc003fem.5; human.;


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