Mouse Folh1 ELISA Kit

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  • Alternative name

    Mouse folate hydrolase 1 ELISA Kit;Mouse folylpoly-gamma-glutamate carboxypeptidase ELISA Kit;Mouse FGCP ELISA Kit;Mouse glutamate carboxypeptidase II ELISA Kit;Mouse GCPII ELISA Kit;Mouse membrane glutamate carboxypeptidase ELISA Kit;Mouse mGCP ELISA Kit;Mouse N-acetylated-alpha-linked acidic dipeptidase I ELISA Kit;Mouse NAALADase I ELISA Kit;Mouse prostate-specific membrane antigen homolog ELISA Kit;Mouse pteroylpoly-gamma-glutamate carboxypeptidase ELISA Kit;Mouse mopsm ELISA Kit;Mouse Naalad1 ELISA Kit;Mouse GCP2 ELISA Kit;Mouse glutamate carboxypeptidase 2 ELISA Kit;

  • Catalog
    E054326
  • species
    Mouse
  • GeneFolh1
  • Standard CurveMouse Folh1 ELISA Kit
  • Other Species Human FOLH1 ELISA KitPorcine FOLH1 ELISA KitRat Folh1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FOLH1. No significant cross-reactivity or interference between FOLH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FOLH1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Folh1 ELISA Kit allows for the in vitro quantitative determination of Folh1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: FOLH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOLH1 antibody and an FOLH1-HRP conjugate. The assay sample and buffer are incubated together with FOLH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOLH1 concentration since FOLH1 from samples and FOLH1-HRP conjugate compete for the anti-FOLH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOLH1 from the sample, fewer sites are left to bind FOLH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOLH1 concentration in each sample is interpolated from this standard curve.
  • Mouse Glutamate carboxypeptidase 2 Protein information
  • Uniprot ID FOLH1_MOUSE
  • Uniprot AC O35409; Q0VDM5; Q9DCC2;
  • UniGene Mm.269137;
  • GeneID 53320
  • KEGG mmu:53320;
  • Mouse Glutamate carboxypeptidase 2 Protein SEQUENCE
  • SEQUENCE 752 AA; 84574 MW; F96041949214E8F3 CRC64;

    MWNALQDRDS AEVLGHRQRW LRVGTLVLAL TGTFLIGFLF GWFIKPSNEA

    TGNVSHSGMK KEFLHELKAE NIKKFLYNFT RTPHLAGTQN NFELAKQIHD

    QWKEFGLDLV ELSHYDVLLS YPNKTHPNYI SIINEDGNEI FKTSLSEQPP

    PGYENISDVV PPYSAFSPQG TPEGDLVYVN YARTEDFFKL EREMKISCSG

    KIVIARYGKV FRGNMVKNAQ LAGAKGMILY SDPADYFVPA VKSYPDGWNL

    PGGGVQRGNV LNLNGAGDPL TPGYPANEHA YRHELTNAVG LPSIPVHPIG

    YDDAQKLLEH MGGPAPPDSS WKGGLKVPYN VGPGFAGNFS TQKVKMHIHS

    YTKVTRIYNV IGTLKGALEP DRYVILGGHR DAWVFGGIDP QSGAAVVHEI

    VRSFGTLKKK GRRPRRTILF ASWDAEEFGL LGSTEWAEEH SRLLQERGVA

    YINADSSIEG NYTLRVDCTP LMYSLVYNLT KELQSPDEGF EGKSLYDSWK

    EKSPSPEFIG MPRISKLGSG NDFEVFFQRL GIASGRARYT KNWKTNKVSS

    YPLYHSVYET YELVVKFYDP TFKYHLTVAQ VRGAMVFELA NSIVLPFDCQ

    SYAVALKKYA DTIYNISMKH PQEMKAYMIS FDSLFSAVNN FTDVASKFNQ

    RLQELDKSNP ILLRIMNDQL MYLERAFIDP LGLPGRPFYR HIIYAPSSHN

    KYAGESFPGI YDALFDISSK VNASKAWNEV KRQISIATFT VQAAAETLRE

    VA

  • UCSC uc009ifh.2; mouse.;


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