Mouse Folh1 ELISA Kit

Alternative name
Mouse folate hydrolase 1 ELISA Kit;Mouse folylpoly-gamma-glutamate carboxypeptidase ELISA Kit;Mouse FGCP ELISA Kit;Mouse glutamate carboxypeptidase II ELISA Kit;Mouse GCPII ELISA Kit;Mouse membrane glutamate carboxypeptidase ELISA Kit;Mouse mGCP ELISA Kit;Mouse N-acetylated-alpha-linked acidic dipeptidase I ELISA Kit;Mouse NAALADase I ELISA Kit;Mouse prostate-specific membrane antigen homolog ELISA Kit;Mouse pteroylpoly-gamma-glutamate carboxypeptidase ELISA Kit;Mouse mopsm ELISA Kit;Mouse Naalad1 ELISA Kit;Mouse GCP2 ELISA Kit;Mouse glutamate carboxypeptidase 2 ELISA Kit;
Catalog
E054326
species
Mouse
GeneFolh1
SpecificityThis assay has high sensitivity and excellent specificity for detection of FOLH1. No significant cross-reactivity or interference between FOLH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FOLH1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Folh1 ELISA Kit allows for the in vitro quantitative determination of Folh1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: FOLH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOLH1 antibody and an FOLH1-HRP conjugate. The assay sample and buffer are incubated together with FOLH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOLH1 concentration since FOLH1 from samples and FOLH1-HRP conjugate compete for the anti-FOLH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOLH1 from the sample, fewer sites are left to bind FOLH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOLH1 concentration in each sample is interpolated from this standard curve.
Mouse Glutamate carboxypeptidase 2 Protein information
Uniprot ID FOLH1_MOUSE
Uniprot AC O35409; Q0VDM5; Q9DCC2;
UniGene Mm.269137;
GeneID 53320
KEGG mmu:53320;
Mouse Glutamate carboxypeptidase 2 Protein SEQUENCE
SEQUENCE 752 AA; 84574 MW; F96041949214E8F3 CRC64;
MWNALQDRDS AEVLGHRQRW LRVGTLVLAL TGTFLIGFLF GWFIKPSNEA
TGNVSHSGMK KEFLHELKAE NIKKFLYNFT RTPHLAGTQN NFELAKQIHD
QWKEFGLDLV ELSHYDVLLS YPNKTHPNYI SIINEDGNEI FKTSLSEQPP
PGYENISDVV PPYSAFSPQG TPEGDLVYVN YARTEDFFKL EREMKISCSG
KIVIARYGKV FRGNMVKNAQ LAGAKGMILY SDPADYFVPA VKSYPDGWNL
PGGGVQRGNV LNLNGAGDPL TPGYPANEHA YRHELTNAVG LPSIPVHPIG
YDDAQKLLEH MGGPAPPDSS WKGGLKVPYN VGPGFAGNFS TQKVKMHIHS
YTKVTRIYNV IGTLKGALEP DRYVILGGHR DAWVFGGIDP QSGAAVVHEI
VRSFGTLKKK GRRPRRTILF ASWDAEEFGL LGSTEWAEEH SRLLQERGVA
YINADSSIEG NYTLRVDCTP LMYSLVYNLT KELQSPDEGF EGKSLYDSWK
EKSPSPEFIG MPRISKLGSG NDFEVFFQRL GIASGRARYT KNWKTNKVSS
YPLYHSVYET YELVVKFYDP TFKYHLTVAQ VRGAMVFELA NSIVLPFDCQ
SYAVALKKYA DTIYNISMKH PQEMKAYMIS FDSLFSAVNN FTDVASKFNQ
RLQELDKSNP ILLRIMNDQL MYLERAFIDP LGLPGRPFYR HIIYAPSSHN
KYAGESFPGI YDALFDISSK VNASKAWNEV KRQISIATFT VQAAAETLRE
VA
UCSC uc009ifh.2; mouse.;

Category

Mouse Folh1 ELISA Kit