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Alternative name
Solute carrier family 2, facilitated glucose transporter member 2 ELISA KIT; Glucose transporter type 2, liver ELISA KIT; GLUT-2Slc2a2 ELISA KIT; Glut-2 ELISA KIT; Glut2 ELISA KIT; GLUT-2 ELISA KIT
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Catalog
E054321
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species
Mouse
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GeneGLUT2
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Other Species
Human GLUT2 ELISA Kit
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SpecificityThis kit recognizes natural and recombinant Mouse GLUT2. No significant cross-reactivity or interference between Mouse GLUT2 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.094ng/mL
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Intended UseMouse GLUT2 ELISA Kit allows for the in vitro quantitative determination of GLUT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse GLUT2 concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GLUT2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GLUT2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GLUT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GLUT2. You can calculate the concentration of GLUT2 in the samples by comparing the OD of the samples to the standard curve.
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