Human ACVR2B ELISA Kit

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  • Alternative name

    Human Activin receptor type IIB ELISA Kit;Human ActR-IIB ELISA Kit;Human ACTRIIB ELISA Kit;Human HTX4 ELISA Kit;Human activin A receptor type 2B ELISA Kit;Human activin receptor type-2B ELISA Kit;Human activin A receptor, type IIB ELISA Kit;

  • Catalog
    E000543
  • species
    Human
  • GeneACVR2B
  • Standard CurveHuman ACVR2B ELISA Kit
  • Other Species Mouse Acvr2b ELISA KitBovine ACVR2B ELISA KitChicken ACVR2B ELISA KitPorcine ACVR2B ELISA KitRat Acvr2b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACVR2B. No significant cross-reactivity or interference between ACVR2B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACVR2B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ACVR2B ELISA Kit allows for the in vitro quantitative determination of ACVR2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: ACVR2B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACVR2B antibody and an ACVR2B-HRP conjugate. The assay sample and buffer are incubated together with ACVR2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACVR2B concentration since ACVR2B from samples and ACVR2B-HRP conjugate compete for the anti-ACVR2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ACVR2B from the sample, fewer sites are left to bind ACVR2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACVR2B concentration in each sample is interpolated from this standard curve.
  • Human Activin receptor type-2B Protein information
  • Uniprot ID AVR2B_HUMAN
  • Uniprot AC Q13705; Q4VAV0;
  • UniGene Hs.174273;
  • GeneID 93
  • KEGG hsa:93;
  • Human Activin receptor type-2B Protein SEQUENCE
  • SEQUENCE 512 AA; 57724 MW; B377FEF92EF74937 CRC64;

    MTAPWVALAL LWGSLCAGSG RGEAETRECI YYNANWELER TNQSGLERCE

    GEQDKRLHCY ASWRNSSGTI ELVKKGCWLD DFNCYDRQEC VATEENPQVY

    FCCCEGNFCN ERFTHLPEAG GPEVTYEPPP TAPTLLTVLA YSLLPIGGLS

    LIVLLAFWMY RHRKPPYGHV DIHEDPGPPP PSPLVGLKPL QLLEIKARGR

    FGCVWKAQLM NDFVAVKIFP LQDKQSWQSE REIFSTPGMK HENLLQFIAA

    EKRGSNLEVE LWLITAFHDK GSLTDYLKGN IITWNELCHV AETMSRGLSY

    LHEDVPWCRG EGHKPSIAHR DFKSKNVLLK SDLTAVLADF GLAVRFEPGK

    PPGDTHGQVG TRRYMAPEVL EGAINFQRDA FLRIDMYAMG LVLWELVSRC

    KAADGPVDEY MLPFEEEIGQ HPSLEELQEV VVHKKMRPTI KDHWLKHPGL

    AQLCVTIEEC WDHDAEARLS AGCVEERVSL IRRSVNGTTS DCLVSLVTSV

    TNVDLPPKES SI

  • UCSC uc003cif.4; human. [Q13705-1];




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