Human ACVR2B ELISA Kit

Alternative name
Human Activin receptor type IIB ELISA Kit;Human ActR-IIB ELISA Kit;Human ACTRIIB ELISA Kit;Human HTX4 ELISA Kit;Human activin A receptor type 2B ELISA Kit;Human activin receptor type-2B ELISA Kit;Human activin A receptor, type IIB ELISA Kit;
Catalog
E000543
species
Human
GeneACVR2B
Standard Curve
Other Species Mouse Acvr2b ELISA Kit Bovine ACVR2B ELISA Kit Chicken ACVR2B ELISA Kit Porcine ACVR2B ELISA Kit Rat Acvr2b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACVR2B. No significant cross-reactivity or interference between ACVR2B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACVR2B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ACVR2B ELISA Kit allows for the in vitro quantitative determination of ACVR2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: ACVR2B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACVR2B antibody and an ACVR2B-HRP conjugate. The assay sample and buffer are incubated together with ACVR2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACVR2B concentration since ACVR2B from samples and ACVR2B-HRP conjugate compete for the anti-ACVR2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ACVR2B from the sample, fewer sites are left to bind ACVR2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACVR2B concentration in each sample is interpolated from this standard curve.
Human Activin receptor type-2B Protein information
Uniprot ID AVR2B_HUMAN
Uniprot AC Q13705; Q4VAV0;
UniGene Hs.174273;
GeneID 93
KEGG hsa:93;
Human Activin receptor type-2B Protein SEQUENCE
SEQUENCE 512 AA; 57724 MW; B377FEF92EF74937 CRC64;
MTAPWVALAL LWGSLCAGSG RGEAETRECI YYNANWELER TNQSGLERCE
GEQDKRLHCY ASWRNSSGTI ELVKKGCWLD DFNCYDRQEC VATEENPQVY
FCCCEGNFCN ERFTHLPEAG GPEVTYEPPP TAPTLLTVLA YSLLPIGGLS
LIVLLAFWMY RHRKPPYGHV DIHEDPGPPP PSPLVGLKPL QLLEIKARGR
FGCVWKAQLM NDFVAVKIFP LQDKQSWQSE REIFSTPGMK HENLLQFIAA
EKRGSNLEVE LWLITAFHDK GSLTDYLKGN IITWNELCHV AETMSRGLSY
LHEDVPWCRG EGHKPSIAHR DFKSKNVLLK SDLTAVLADF GLAVRFEPGK
PPGDTHGQVG TRRYMAPEVL EGAINFQRDA FLRIDMYAMG LVLWELVSRC
KAADGPVDEY MLPFEEEIGQ HPSLEELQEV VVHKKMRPTI KDHWLKHPGL
AQLCVTIEEC WDHDAEARLS AGCVEERVSL IRRSVNGTTS DCLVSLVTSV
TNVDLPPKES SI
UCSC uc003cif.4; human. [Q13705-1];

Category

Human ACVR2B ELISA Kit

Human Activin receptor type IIB ELISA Kit;Human ActR-IIB ELISA Kit;Human ACTRIIB ELISA Kit;Human HTX4 ELISA Kit;Human activin A receptor type 2B ELISA Kit;Human activin receptor type-2B ELISA Kit;Human activin A receptor, type IIB ELISA Kit;