Glucocorticoid receptor ELISA KIT; Nuclear receptor subfamily 3 group C member 1Nr3c1 ELISA KIT; Grl ELISA KIT; Grl1 ELISA KIT; GR ELISA KIT
Catalog
E054223
species
Mouse
GeneGR-alpha
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse GR-alpha. No significant cross-reactivity or interference between mouse GR-alpha and analogues was observed.
SensitivityThe minimum detectable dose of mouse GR-alpha is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseMouse GR-alpha ELISA Kit allows for the in vitro quantitative determination of GR-alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GR-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GR-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GR-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GR-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
No manual or datasheet available for this product.