Intended UseHuman BP230-AB ELISA Kit allows for the in vitro quantitative determination of BP230-AB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human BP230-AB in samples. Add sample to wells pre-coated with one Human Bullous Pemphigoid 230 antigen, at same time add HRP-conjugated Human Bullous Pemphigoid 230 antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human BP230-AB in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.