SpecificityThis assay has high sensitivity and excellent specificity for detection of human Bullous Pemphigoid (BP)antibody (IgG). No significant cross-reactivity or interference between human Bullous Pemphigoid (BP)antibody (IgG) and analogues was observed.
Intended UseHuman DST ELISA Kit allows for the in vitro quantitative determination of DST , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Bullous Pemphigoid (BP)antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.