Mouse Gfap ELISA Kit

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  • Alternative name

    Mouse GFAP ELISA Kit;Mouse AI836096 ELISA Kit;Mouse glial fibrillary acidic protein ELISA Kit;Mouse intermediate filament protein ELISA Kit;

  • Catalog
    E054108
  • species
    Mouse
  • GeneGfap
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GFAP. No significant cross-reactivity or interference between Mouse GFAP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GFAP and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.375pg/mL
  • Intended UseMouse Gfap ELISA Kit allows for the in vitro quantitative determination of Gfap , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GFAP in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GFAP. You can calculate the concentration of Mouse GFAP in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Glial fibrillary acidic protein Protein information
  • Uniprot ID GFAP_MOUSE
  • Uniprot AC Q7TQ30; Q80VX6; Q925K2; Q925K3;
  • UniGene Mm.1239;
  • GeneID 14580
  • KEGG mmu:14580;
  • Mouse Glial fibrillary acidic protein Protein SEQUENCE
  • SEQUENCE 430 AA; 49900 MW; C0FB500AE1588377 CRC64;

    MERRRITSAR RSYASETVVR GLGPSRQLGT MPRFSLSRMT PPLPARVDFS

    LAGALNAGFK ETRASERAEM MELNDRFASY IEKVRFLEQQ NKALAAELNQ

    LRAKEPTKLA DVYQAELREL RLRLDQLTAN SARLEVERDN FAQDLGTLRQ

    KLQDETNLRL EAENNLAAYR QEADEATLAR VDLERKVESL EEEIQFLRKI

    YEEEVRELRE QLAQQQVHVE MDVAKPDLTA ALREIRTQYE AVATSNMQET

    EEWYRSKFAD LTDAASRNAE LLRQAKHEAN DYRRQLQALT CDLESLRGTN

    ESLERQMREQ EERHARESAS YQEALARLEE EGQSLKEEMA RHLQEYQDLL

    NVKLALDIEI ATYRKLLEGE ENRITIPVQT FSNLQIRETS LDTKSVSEGH

    LKRNIVVKTV EMRDGEVIKD SKQEHKDVVM

  • UCSC uc007lsx.2; mouse. [P03995-2];



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