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Mouse Gclm ELISA Kit

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  • Alternative name

    Mouse GCS light chain ELISA Kit;Mouse gamma-ECS regulatory subunit ELISA Kit;Mouse gamma-glutamylcysteine synthetase regulatory subunit ELISA Kit;Mouse Glutamate--cysteine ligase modifier subunit ELISA Kit;Mouse Glclr ELISA Kit;Mouse AI649393 ELISA Kit;Mouse Gcmc ELISA Kit;Mouse glutamate-cysteine ligase, modifier subunit ELISA Kit;Mouse glutamate--cysteine ligase regulatory subunit ELISA Kit;
  • Catalog
    E054027
  • species
    Mouse
  • GeneGclm
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GCLM. No significant cross-reactivity or interference between Mouse GCLM and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GCLM and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Gclm ELISA Kit allows for the in vitro quantitative determination of Gclm , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GCLM in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GCLM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GCLM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GCLM. You can calculate the concentration of Mouse GCLM in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Glutamate--cysteine ligase regulatory subunit Protein information
  • Uniprot ID GSH0_MOUSE
  • Uniprot AC O09172;
  • UniGene Mm.292676;
  • GeneID 14630
  • KEGG mmu:14630;
  • Mouse Glutamate--cysteine ligase regulatory subunit Protein SEQUENCE

  • SEQUENCE 274 AA; 30535 MW; E1D591CAB71CEA11 CRC64;

    MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT

    LNEWSSQISP DLVREFPDVL ECTMSHAVEK INPDEREEMK VSAKLFIVGS

    NSSSSTRSAV DMACSVLGVA QLDSVIMASP PIEDGVNLSL EHLQPYWEEL

    ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL

    TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIEAQ DWVPLWLLRY

    SVIVKSRGII KSKGYILQAK RRGS

  • UCSC uc008reo.2; mouse.;



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